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Test ID: HSEP    
Hereditary Spherocytosis Evaluation

Specimen Type Describes the specimen type needed for testing

Whole Blood EDTA

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

See Recommendations for Collection of Control Specimens for FRAG, HSEP, and HAEVP in Special Instructions.



1. Hereditary Spherocytosis Information Sheet (Supply T706) is available in Special Instruction.

2. If not ordering electronically, please submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.


Specimens must arrive in the testing laboratory within 96 hours of draw.


A whole blood EDTA specimen and a control specimen are required.



Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Immediately refrigerate specimen after draw.

2. Do not transfer blood to other containers.

3. Rubber band patient specimen and control vial together.

Additional Information: Patient's age and sex are required.


Normal Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen at the same time from a normal, unrelated, non-smoking individual.

2. Clearly write normal control on outermost label and also indicate sex of control on same label.

3. Immediately refrigerate specimen after draw.

4. Do not transfer blood to other containers.

5. Rubber band control vial and patient specimen together.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

2 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject







Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
ControlRefrigerated4 days
Whole Blood EDTARefrigerated4 days