Enteric Pathogens Culture, Stool
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, the reflex tests may be performed and charged separately. Includes Aeromonas, Campylobacter, Salmonella, Shigella, and Yersinia.
Note: Does not include culture for Escherichia coli O157:H7.
See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.
Specimen Type Describes the specimen type needed for testing
1. If Vibrio is suspected, order VIBC / Vibrio Culture, Stool.
2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC. Pathogens for which susceptibility testing would be pursued are: Shigella species, Yersinia species, Campylobacter species, Arcobacter species, and Vibrio species. If susceptibilities are not appropriate they will not be performed, ZMMLS will be canceled at report time.
3. Clostridium difficile, a major cause of nosocomial diarrhea, is not detected by this test (order CDFRP / Clostridium difficile Toxin, Molecular Detection, PCR, Feces).
4. Escherichia coli O157:H7 is not detected by this test (Order STFRP / Shiga Toxin, Molecular Detection, PCR, Feces).
Specimen must arrive within 96 hours of collection.
Specimen source is required.
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Patient Preparation: Medications: Do not use antacids, barium, bismuth, antidiarrheal medication or oily laxatives before collection of specimen.
Supplies: C and S vial (T058)
Specimen Type: Preserved Stool
Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S [T058])
Specimen Volume: Representative portion of stool specimen
1. Collect fresh stool and submit 1 gram or 5 mL in container with transport medium.
2. Place stool in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Specimen >96 hours, unpreserved stool, ECOFIX preservative, formalin, or PVA fixative
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Fecal||Ambient (preferred)||4 days|