BPRP - Specimen: Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR

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Test ID: BPRP    
Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.

Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

General Request Form (T239) (http://www.mayomedicallaboratories.com/it-mmfiles/general-request-form.pdf)


Specimen source is required.


Submit only 1 of the following specimens:



Specimen Type: Nasopharyngeal swab

Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as Stuart's with or without charcoal, or Amies with or without charcoal (Transwab Nasopharyngeal with Charcoal System [T286]) or a green top nasopharyngeal swab (rayon mini-tip) with Stuart’s media (no charcoal) (T515)

Additional Information: Other swab or media types may be inhibitory to PCR testing and will be rejected.



Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash

Container/Tube: Sterile container with a screw top cap (no transport media)

Specimen Volume: Entire collection

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Nose, nasal, or throat swab; calcium alginate or cotton-tipped swab; swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media; ESwab; swabs with solid plastic shaft; dry swab

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Ambient 7 days
 Frozen 7 days