Tropheryma whipplei, Molecular Detection, PCR
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results.
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Tropheryma whipplei DNA is not likely.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Submit only 1 of the following specimens:
Specimen Type: Biopsy
Sources: Small intestine tissue (duodenum, ileum, or jejunum), brain, gastrointestinal tissue, heart valve, lymph node, synovial tissue, or other visceral tissue
Preferred: Fresh tissue
Acceptable: Tissue fixed in a paraffin block
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Stability Information:
Fresh tissue: Frozen (preferred)/Refrigerated <24 hours
Paraffin block: Ambient (preferred)/Refrigerated
Specimen Type: Fluid
Sources: Spinal, synovial, or vitreous humor
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated (preferred)/Frozen
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Spinal Fluid, Synovial Fluid, or Vitreous Humor Fluid: 0.5 mL/Tissue: 5 mm
Green top (heparin) tube
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.