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Test ID: TWRP    
Tropheryma whipplei, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results.

 

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Tropheryma whipplei DNA is not likely.

 

Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.

 

Submit only 1 of the following specimens:

 

Specimen Type: Biopsy

Sources: Small intestine tissue (duodenum, ileum, or jejunum), brain, gastrointestinal tissue, heart valve, lymph node, synovial tissue, or other visceral tissue

Preferred: Fresh tissue

Acceptable: Tissue fixed in a paraffin block

Container/Tube: Sterile container

Specimen Volume: Entire collection

Specimen Stability Information:

Fresh tissue: Frozen (preferred) <7 days/Refrigerated <24 hours

Paraffin block: Ambient (preferred)/Refrigerated

 

Specimen Type: Fluid

Sources: Spinal, synovial, or vitreous humor

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated (preferred) <24 hours/Frozen <7 days

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Spinal Fluid, Synovial Fluid, or Vitreous Humor Fluid: 0.5 mL/Tissue: 5 mm

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Green top (heparin) tube

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesVaries