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Test ID: LHSV    
Herpes Simplex Virus (HSV), Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Body or ocular fluid

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by HSV DNA is not likely.

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Specimen Type: Genital

Sources: Cervix, rectum, urethra, vagina, other genital site

Container/Tube: BBL CultureSwab (Supply T092)

Specimen Volume: Swab

Collection Instructions: Place swab back into swab cylinder.

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

Specimen Stability Information: Refrigerated (preferred)/Frozen

Additional Information: The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by HSV DNA is not likely.

 

Specimen Type: Swab

Sources:

Preferred: Dermal, eye, throat

Acceptable: Anal/rectal, urethral

Container/Tube: BBL CultureSwab (Supply T092)

Specimen Volume: Swab

Collection Instructions: Place swab back into swab cylinder.

Specimen Stability Information: Refrigerated (preferred)/Frozen

 

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Container/Tube: Sterile container containing 1 to 2 mL of sterile saline or multi-microbe medium (M5) (Supply T484)

Specimen Volume: Entire collection

Collection Instructions: Collect a fresh tissue specimen.

 

Acceptable:

Specimen Type: Amniotic fluid

Container/Tube: Amniotic fluid container

Specimen Volume: 0.5 mL

 

Specimen Type: Urine (<1 month old infant)

Container/Tube: Sterile container      

Specimen Volume: 0.5 mL

 

Specimen Type: Whole blood (<1 month old infant)

Container/Tube: EDTA

Specimen Volume: 0.5 mL

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Body/Ocular Fluid or Spinal Fluid: 0.3 mL/Respiratory Specimen: 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and/or paraffin-embedded tissues

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesVaries7 days