Test ID: LHSV
Herpes Simplex Virus (HSV), Molecular Detection, PCR
Specimen Type
Describes the specimen type needed for testing
Specimen Required
Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen source is required.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Body or ocular fluid
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by HSV DNA is not likely.
Specimen Stability Information: Refrigerated (preferred)/Frozen
Specimen Type: Genital
Sources: Cervix, rectum, urethra, vagina, other genital site
Container/Tube: BBL CultureSwab (Supply T092)
Specimen Volume: Swab
Collection Instructions: Place swab back into swab cylinder.
Specimen Stability Information: Refrigerated (preferred)/Frozen
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Specimen Stability Information: Refrigerated (preferred)/Frozen
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Stability Information: Refrigerated (preferred)/Frozen
Additional Information: The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by HSV DNA is not likely.
Specimen Type: Swab
Sources:
Preferred: Dermal, eye, throat
Acceptable: Anal/rectal, urethral
Container/Tube: BBL CultureSwab (Supply T092)
Specimen Volume: Swab
Collection Instructions: Place swab back into swab cylinder.
Specimen Stability Information: Refrigerated (preferred)/Frozen
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Container/Tube: Sterile container containing 1 to 2 mL of sterile saline or multi-microbe medium (M5) (Supply T484)
Specimen Volume: Entire collection
Collection Instructions: Collect a fresh tissue specimen.
Acceptable:
Specimen Type: Amniotic fluid
Container/Tube: Amniotic fluid container
Specimen Volume: 0.5 mL
Specimen Type: Urine (<1 month old infant)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Type: Whole blood (<1 month old infant)
Container/Tube: EDTA
Specimen Volume: 0.5 mL
Specimen Minimum Volume
Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and/or paraffin-embedded tissues |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |


