Test ID: SPN
Pneumocystis Smear
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Specimen source is required.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Bronchoalveolar lavage
Container/Tube: Sterile container
Specimen Volume: Minimum of 2 mL
Specimen Type: Lung or open lung tissue
Container/Tube: Sterile container
Specimen Volume: Minimum of a rice size piece
Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.
Alternate:
Specimen Type: Bronchial washing, sputum, or tracheal secretion
Container/Tube: Sterile container
Specimen Volume: Minimum of 1 mL
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Brochoalveolar lavage: 2mL
Sputum, bronchial washings, and tracheal secretions: 1 mL
Lung tissue and open lung biopsy: rice-sized piece of tissue
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Fixed tissue; viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth); wood shaft or charcoal swab |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
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