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Unit Code 80401:
Acanthamoeba/Naegleria species, Corneal Scrapings or Spinal Fluid

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Specimen Required

The preferred specimens for Acanthamoeba culture

are corneal scraping and biopsy. Swabs are suboptimal

and may result in a falsely negative result. 

 

Submit only 1 of the following specimens:

 

Central Nervous System Tissue, Contact Lens, Corneal

Scrapings, or Corneal Tissue

Place lens, scrapings, or tissue in 1 mL of sterile saline or minimal

essential medium.

Note:    1.    Specimen source is required on request form for

                      processing.

                  2. If ordering electronically, no form is required with the

                      specimen. If not ordering electronically, please complete

                      and submit a "Microbiology Request Form" (Supply T244)

                      with the specimen.

                   

Contact Lens Solution

Submit at least 1 mL of contact lens solution in a screw-capped,

sterile container. Maintain sterility and forward promptly.

Note:    1.    Specimen source is required on request form for

                      processing.

                  2. If ordering electronically, no form is required with the

                      specimen. If not ordering electronically, please complete

                      and submit a "Microbiology Request Form" (Supply T244)

                      with the specimen.

                   

Spinal Fluid

1 mL of spinal fluid.

Note:    1.    Specimen source is required on request form for

                      processing.

                  2. If ordering electronically, no form is required with the

                      specimen. If not ordering electronically, please complete

                      and submit a "Microbiology Request Form" (Supply T244)

                      with the specimen.

Transport Temperature

Ambient\Refrig NO\Frozen NO

Reject Due To

Specimens Other Than:                 CSF, corneal scrapings and tissue,

                                                                contact lens/solution, CNS tiss

Anticoagulants Other Than:           NA

Hemolysis:                                          NA

Thawing:                                             

Lipemia:                                               NA

Day(s) and Time(s) Test Performed

Monday through Sunday; Continuously


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