Test ID: ACANT
Acanthamoeba/Naegleria species, Corneal Scraping or Spinal Fluid
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Central nervous system tissue
Container/Tube: Sterile container with 1 mL of saline or minimal essential medium
Specimen Volume: Entire collection
Specimen Stability Information: Ambient
Specimen Type: Contact lens, corneal scraping, corneal tissue
Container/Tube: Sterile container with 1 mL of saline or minimal essential medium
Specimen Volume: Entire collection
Additional Information: The preferred specimen for Acanthamoeba culture from an ocular source is corneal scraping or biopsy. Swab is suboptimal and may result in a falsely negative result. Supplementary slides may be submitted in addition to the scraping or biopsy, but slides alone will not be accepted for testing.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Contact lens solution
Container/Tube: Sterile container
Specimen Volume: 2 mL
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 1 mL
Specimen Stability Information: Ambient
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Slides only received and samples received on wood swabs |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
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