Test ID: PFGE
Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)
Specimen Type
Describes the specimen type needed for testing
Specimen Required
Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube: Agar slant
Specimen Volume: Isolates on separate agar slants
Collection Instructions:
1. Individually bag each pulsed-field gel electrophoresis (PFGE) specimen, then place all PFGE specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.
2. Label specimen as an etiologic agent.
Additional Information:
1. Specimen source and organism identification are required.
2. Each slant must be submitted under a separate order.
3. Original isolates should be saved at the client site if additional PFGE testing will be needed.
4. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||


