PFGE - Specimen: Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Test Catalog

Test ID: PFGE    
Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Specimen Type Describes the specimen type needed for testing

Varies

Advisory Information

This test identifies bacterial strains, except for Staphylococcus aureusStaphylococcus spp., not S. aureus; Listeria spp., Bacillus spp., Enterococcus spp., Streptococcus spp., Lactobacillus spp., Legionella spp., Enterobacteriaceae, non-Enterobacteriaceae, and Clostridium perfringens.

 

For Staphylococcus aureus isolates, order BTWGS / Bacterial Typing by Whole Genome Sequencing.

Shipping Instructions

See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Necessary Information

Specimen source and organism identification are required for processing.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.

Additional Information:

1. Each slant must be submitted under a separate order.

2. Original isolates should be saved at the client site if additional PFGE testing may be needed (eg, to compare to future outbreak isolates that may occur).

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)
 Refrigerated 
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