PFGE - Specimen: Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

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Test ID: PFGE    
Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.

Additional Information:

1. Specimen source and organism identification are required for processing.

2. Each slant must be submitted under a separate order.

3. Original isolates should be saved at the client site if additional PFGE testing may be needed.

4. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected









Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)