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Test ID: PFGE    
Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants

Collection Instructions:

1. Individually bag each pulsed-field gel electrophoresis (PFGE) specimen, then place all PFGE specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.

2. Label specimen as an etiologic agent/infectious substance.

Additional Information:

1. Specimen source and organism identification are required.

2. Each slant must be submitted under a separate order.

3. Original isolates should be saved at the client site if additional PFGE testing may be needed.

4. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)
 Refrigerated