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Unit Code 80337:
Galactose-1-Phosphate (Gal-1-P), Erythrocytes

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Specimen Required

Draw blood in a green-top (heparin) tube(s), and process a minimum of

2 ml of heparinized whole blood as follows:

1. Immediately centrifuge for 10 minutes at 2,000 rpm.

2. Discard the plasma and buffy coat layers.

3. Add a cold 0.9% saline solution to the erythrocytes (about

     2 times the volume of erythrocytes).

4. Mix gently by inversion and centrifuge again for 10 minutes

     at 2,000 rpm.

5. Remove and discard the saline.

6. Repeat the wash steps (3-5) 2 more times.

7. After the final centrifugation, remove and discard the saline and a

     thin layer of the top cells.

8. Immediately freeze the washed, packed cells (red cell pellet from

     step 7) and send specimen frozen in plastic vial.

Transport Temperature

Frozen\Refrig NO\Ambient NO

Reject Due To

Specimens Other Than:                 Frozen washed erythrocytes

Anticoagulants Other Than:           Heparin

Hemolysis:                                          NA

Thawing:                                              Yes

Lipemia:                                               NA

Day(s) and Time(s) Test Performed

Tuesday; Thursday 8:00 a.m.

Key