Galactose-1-Phosphate (Gal-1-P), Erythrocytes
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Erythrocytes must be washed within 4 hours of draw.
Collection Container/Tube: Green top (heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: Washed, packed cells (red cell pellet from step f in Collection Instructions)
1. Collect and process a minimum of 2 mL of whole blood as follows:
a. Immediately centrifuge for 10 minutes at 650 x G. For conversion to RPMs, see Additional Information.
b. Discard the plasma and buffy coat layers.
c. Add a cold 0.9% saline solution to the erythrocytes (about 2 times the volume of erythrocytes).
d. Mix gently by inversion and centrifuge again for 10 minutes at 650 x G.
e. Remove and discard the saline.
f. Repeat the wash steps (steps c-e) 2 more times.
2. After the final centrifugation, remove and discard the saline and a thin layer of the top cells.
The relative centrifugal force (G-force) can be estimated by applying the following formula: g=11.18 x r x (n/1000)2.
Where: r=radius in centimeters and n=speed in RPM.
The radius from the center of the rotation axis to the bottom or outermost portion of the test tube should be used. RCF is expressed relative to the force of the earth's gravity.
Forms: If not ordering electronically, submit a Biochemical Genetics Request Form (Supply T439) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Red cell pellet from heparinized whole blood
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Washed RBC||Frozen||9 days|