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Unit Code 80333:
Fibroblast Culture for Genetic Testing

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Specimen Required

Please provide a reason for referral with each specimen. The laboratory

will not reject testing if this information is not provided, but appropriate

testing and interpretation may be compromised or delayed.

 

Submit only 1 of the following specimens:

 

Autopsy

Wash biopsy site with an antiseptic soap (e.g., pHisoHex). Thoroughly

rinse area with sterile water. Do not use alcohol or iodine preparations.  

Biopsy specimens are best taken by punch biopsy to include full

thickness of dermis. Biopsy should be 4 mm in diameter. Aseptically

place biopsy in a screw-capped, sterile container with sterile Hank's

balanced salt solution, Ringer's solution, or normal saline. (Hank's

balanced salt solution [Supply T132] is supplied.) Do not handle

with hands. Label container with patient's name and laboratory

control number. Send specimen refrigerated. Specimen cannot be

frozen. Advise Express Mail or equivalent if not on courier service.

Note:    If ordering electronically, please complete and submit a

             "MayoConnect Additional Test Information Form" (Supply

             T357) with the specimen. If not ordering electronically,

             please complete and submit a "Cytogenetics/AFP

             Congenital Disorders Request Form" (Supply T238) with

             the specimen.

 

Chorionic Villi

Obtain 20 mg to 30 mg of chorionic villus specimen (CVS) by the

transabdominal or transcervical method. Transfer the CVS to a

Petri dish containing transport medium. Using a stereomicroscope

and sterile forceps, assess the quality and quantity of the villi and

remove any blood clots and maternal decidua. Transfer the CVS

by using sterile technique to one or two 15-mL centrifuge tube(s)

with 15 mL of transport medium. (Petri dishes and transport medium

[Supply T095] are supplied.) Label the centrifuge tube(s) with

patient's name and laboratory control number. Send specimen

refrigerated. Specimen cannot be frozen. Advise

Express Mail or equivalent if not on courier service.   

Note:    If ordering electronically, please complete and submit a

             "MayoConnect Additional Test Information Form" (Supply

             T357) with the specimen. If not ordering electronically,

             please complete and submit a "Cytogenetics/AFP

             Congenital Disorders Request Form" (Supply T238)

             with the specimen.

 

Products of Conception or Stillbirth

Where a fetus is identified, obtain about 1 cm(3) of placenta

(including 20-mg chorionic villi) and a 1-cm(3) biopsy specimen of

muscle/fascia from the thigh. Do not send the entire fetus. If a fetus

cannot be specifically identified, collect 50-mg villus material or tissue

that appears to be of fetal origin. Do not handle with hands. Aseptically

place each specimen in a screw-capped, sterile container with sterile

Hank's balanced salt solution, Ringer's solution, or normal saline.

(Hank's balanced salt solution [Supply T132] is supplied.)

Label each container with the specimen type, patient's name, and

laboratory control number. Send specimen refrigerated. Specimen

cannot be frozen. Advise Express Mail or equivalent if not on

courier service.

Note:    1. If a fetus is sent, please provide a parental release

                 form or complete the "Final Disposition of Fetal/

                 Stillborn Remains" form and send it with the fetus.

                 (A copy of this form can be found in Special Instructions.)

                 Please note that completion of the form requires a parent's

                 signature.

             2. If ordering electronically, please complete and submit

                 a "MayoConnect Additional Test Information Form"

                 (Supply T357) with the specimen. If not ordering

                 electronically, please complete and submit a "Cytogenetics/

                 AFP Congenital Disorders Request Form" (Supply T238)

                 with the specimen.

 

Skin Biopsy

Wash biopsy site with an antiseptic soap (e.g., pHisoHex).

Thoroughly rinse area with sterile water. Do not use alcohol or iodine

preparations. A local anesthetic may be used. Biopsy specimens

are best taken by punch biopsy to include full thickness of dermis.

Biopsy should be 4 mm in diameter. Aseptically place biopsy in a

screw-capped, sterile container with sterile Hank's balanced salt

solution, Ringer's solution, or normal saline. (Hank's balanced salt

solution [Supply T132] is supplied). Do not handle with hands. Label

container with patient's name and laboratory control number. Send

specimen refrigerated. Specimen cannot be frozen.

Advise Express Mail or equivalent if not on courier service.  

Note:    If ordering electronically, please complete and submit a

             "MayoConnect Additional Test Information Form" (Supply

             T357) with the specimen. If not ordering electronically,

             please complete and submit a "Cytogenetics/AFP

             Congenital Disorders Request Form" (Supply T238) with

             the specimen.

Transport Temperature

Refrig\Ambient OK\Frozen NO

Reject Due To

Specimens Other Than:                 CVS, Tissue, Products of Conception,

                                                                Stillbirth, Autopsy

Anticoagulants Other Than:           NA

Hemolysis:                                          NA

Thawing:                                              NA

Lipemia:                                               NA

 

Day(s) and Time(s) Test Performed

Monday through Friday; 6am - 9pm.  Saturday/Sunday; 6am - 4pm

Key