|Values are valid only on day of printing.|
This request will be processed as a consultation case. An interpretation, which includes an evaluation of the specimen and determination of a diagnosis, will be provided by staff hematopathologists within a formal pathology report to the referring pathologist.
-This test should be ordered when submitting any wet tissue or liquid specimen along with a request for a Hematopathology consultation
-This test should be used when all processing of a formalin-fixed biopsy core is to be performed by Mayo Clinic.
-This test can be used when requesting that a Mayo Hematopathologist provides full bone marrow workup, including appropriate ancillary testing, interpretation, and reporting. This could include processing of the formalin-fixed biopsy as above or use of a paraffin-embedded bone marrow biopsy block, as long as a wet tissue or liquid specimen is also submitted.
-Appropriate additional stains and other ancillary testing may be performed in order to render an accurate diagnosis. The additional stains and tests ordered will have the results included within the final pathology report and will be charged separately.
-If requesting a peripheral blood smear evaluation only, order SPSM / Morphology Evaluation (Special Smear).
-If requesting a Hematopathology consultation on paraffin-embedded tissue and slides only, order 70012 / Pathology Consultation.
-If requesting stand-alone specific testing, please refer to the MML catalog for order codes.
-Flow cytometry on blood or bone marrow order LCMS / Leukemia/Lymphoma Immunophenotyping by Flow Cytometry
-Flow cytometry for tissue order LLPT / Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue
-Chromosome analysis, order CHRBM / Chromosome Analysis, Hematologic Disorders, Bone Marrow
Note: Testing requested by the referring physician (immunostains, molecular studies, etc) may not be performed if deemed unnecessary by the reviewing Mayo Hematopathologist. For all pathology consultations, ancillary testing necessary to determine a diagnosis is ordered at the discretion of the Mayo pathologist.
See Pathology Consultation Ordering Algorithm in Special Instructions.
1. Hematopathology Patient Information (T676) in Special Instructions
2. If not ordering electronically, complete, print, and send Hematopathology/Cytogenetics Test Request Form (T726) (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Specimens needed to provide a complete pathology consultation:
1. Recent peripheral blood smear with CBC report
2. Bone marrow biopsy and clot (unprocessed)
3. Bone marrow aspirate (stained and unstained slides, and liquid)
4. All pending and finalized reports for ancillary testing on above specimens
All specimens, patient history, and requests should be clearly labeled with correct patient information, and case number. Collection date and patient date of birth are required. All specimens must be labeled with specimen type. Although a complete pathology report is not expected a brief history and patient information is required. Please use the Hematopathology Request form.
Specimen Type: Bone marrow aspirate slides
Container/Tube: Transport in plastic slide holders
Preferred: Fresh prep slides made at the time of sample collection
Acceptable: Slides made from anticoagulated sample
1. Prepare slides of bone marrow aspirate immediately after collection or prepare slides from bone marrow aspirate in EDTA within 2 hours of collection.
2. If bone marrow units are sparse or absent or aspirate is a dry tap, make biopsy touch prep slides.
3. Air dry slides.
4. Send 5 slides unfixed/unstained.
5. Place Parafilm around the slide carriers holding unstained slides to prevent exposure to formalin fumes during transport and place slides in a separate bag apart from any formalin-fixed clot or core biopsy specimens during transport. If using slide carriers, make sure they have not been used to carry fixed slides previously.
Specimen Type: Bone marrow aspirate in anticoagulant for possible ancillary testing
Container/Tube: EDTA, heparin, or ACD
Specimen Volume: 5 mL in EDTA, 2-5 mL in ACD, or 2-3 mL in heparin tube
1. Aspirate per standard bone marrow collection procedure.
2. Do not transfer to other tubes or containers.
Specimen Type: Bone marrow core biopsy
Container/Tube: Fixed biopsy core in saline solution for transport or fixed biopsy core embedded in paraffin block
1. Process and embed core in paraffin if desired.
2. If processing at Mayo is desired, place biopsy core in 10% formalin immediately after collection.
3. Fix in 10% formalin for 1 to 2 hours.
4. Transfer core to container with saline, volume sufficient to cover core.
Specimen Type: Bone marrow aspirate clot
Container/Tube: Bone marrow clot in 10% formalin or fixed clot embedded in paraffin block
1. Place 0.5 mL bone marrow aspirate in clot tube.
2. After clot has formed, place clot in 10% formalin.
3. If embedding in paraffin, fix specimen, process and embed per your standard operating procedures.
4. If submitting to Mayo for possible processing and embedding, submit in container of 10% formalin.
Specimen Type: Peripheral blood slides and CBC
Container/Tube: Transport in plastic slide holders.
Preferred: Fresh prep fingerstick slide; include CBC values
Acceptable: Slides made from whole blood in EDTA, made within 8 hours of collection
1. Prepare good quality smear of even thickness from fingerstick.
2. Alternately prepare good quality smear from EDTA vial within 8 hours of collection.
3. Submit unstained and unfixed slides.
No specimen should be rejected.