|Values are valid only on day of printing.|
1. Hematopathology Patient Information Sheet (Supply T676) in Special Instructions
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Specimens needed to provide a complete pathology consultation:
1. Recent peripheral blood smear with CBC report
2. Bone marrow biopsy and clot (unprocessed)
3. Bone marrow aspirate (stained and unstained slides, and liquid)
4. All pending and finalized reports for ancillary testing on above specimens
All specimens, patient history, and requests should be clearly labeled with correct patient information, and case number. Collection date and patient date of birth are required. All specimens must be labeled with specimen type. Although a complete pathology report is not expected, a preliminary pathology report is required in order to ensure that the specimen is known to a referring pathologist. The name and phone number of the ordering pathologist and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.
Specimen Type: Bone marrow aspirate slides
Container/Tube: Transport in plastic slide holders
Preferred: Fresh prep slides made at the time of sample collection
Acceptable: Slides made from anticoagulated sample
1. Prepare slides of bone marrow aspirate immediately after collection or prepare slides from bone marrow aspirate in EDTA within 2 hours of collection.
2. If bone marrow units are sparse or absent or aspirate is a dry tap, make biopsy touch prep slides.
3. Air dry slides.
4. Send 5 slides unfixed/unstained.
5. Place Parafilm around the slide carriers holding unstained slides to prevent exposure to formalin fumes during transport and place slides in a separate bag apart from any formalin-fixed clot or core biopsy specimens during transport. If using slide carriers, make sure they have not been used to carry fixed slides previously.
Specimen Type: Bone marrow aspirate in anticoagulant for possible ancillary testing
Container/Tube: EDTA, heparin, or ACD
Specimen Volume: 5 mL in EDTA, 2-5 mL in ACD, or 2-3 mL in heparin tube
1. Aspirate per standard bone marrow collection procedure.
2. Do not transfer to other tubes or containers.
Specimen Type: Bone marrow core biopsy
Container/Tube: Fixed biopsy core in saline solution for transport or fixed biopsy core embedded in paraffin block
1. Process and embed core in paraffin if desired.
2. If processing at Mayo is desired, place biopsy core in 10% formalin immediately after collection.
3. Fix in 10% formalin for 1 to 2 hours.
4. Transfer core to container with saline, volume sufficient to cover core.
Specimen Type: Bone marrow aspirate clot
Container/Tube: Bone marrow clot in 10% formalin or fixed clot embedded in paraffin block
1. Place 0.5 mL bone marrow aspirate in clot tube.
2. After clot has formed, place clot in 10% formalin.
3. If embedding in paraffin, fix specimen, process and embed per your standard operating procedures.
4. If submitting to Mayo for possible processing and embedding, submit in container of 10% formalin.
Specimen Type: Peripheral blood slides and CBC
Container/Tube: Transport in plastic slide holders.
Preferred: Fresh prep fingerstick slide; include CBC values
Acceptable: Slides made from whole blood in EDTA, made within 8 hours of collection
1. Prepare good quality smear of even thickness from fingerstick.
2. Alternately prepare good quality smear from EDTA vial within 8 hours of collection.
3. Submit unstained and unfixed slides.
Note: No specimen should be rejected. If specimen not received at appropriate temperature or in wrong anticoagulant, include note to laboratory. If questions, contact laboratory.