Hematopathology Consultation, Wet Tissue
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: Hematopathology Patient Information Sheet (Supply T676) in Special Instructions
All specimens, patient history, and requests should be clearly labeled with correct patient information, and case number. Collection date and patient date of birth are required. All specimens must be labeled with specimen type. Although a complete pathology report is not expected, a preliminary pathology report is preferred, but not required in order to ensure that the specimen is known to a referring pathologist. The name and phone number of the ordering pathologist and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.
Bone marrow aspirate slides, bone marrow biopsy and/or peripheral blood slides are required. Bone marrow aspirate in tubes is preferred. Bone marrow aspirate clot may be included.
Specimen Type: Bone marrow aspirate slides
Container/Tube: Transport in plastic slide holders
Preferred: Fresh prep slides made at the time of sample collection
Acceptable: Slides made from anticoagulated sample
1. Prepare slides of bone marrow aspirate immediately after collection or prepare slides from bone marrow aspirate in EDTA within 2 hours of collection.
2. If bone marrow units are sparse/absent or aspirate is a dry tap, make biopsy touch prep slides.
3. Air dry slides.
4. Send slides unfixed/unstained, or stained with Wright-Giemsa.
Specimen Type: Bone marrow aspirate in anticoagulant for possible ancillary testing
Container/Tube: EDTA, heparin, or ACD
Specimen Volume: 5 mL in EDTA, 2-5 mL in ACD, or 2-3 mL in heparin tube
1. Aspirate per standard bone marrow collection procedure.
2. Do not transfer to other tubes or containers.
Specimen Type: Bone marrow core biopsy
Container/Tube: Fixed biopsy core in saline solution for transport or fixed biopsy core embedded in paraffin block
1. Process and embed core in paraffin if desired.
2. If processing at Mayo is desired, place biopsy core in 10% formalin immediately after collection.
3. Fix in 10% formalin for 1 to 2 hours.
4. Transfer core to container with saline, volume sufficient to cover core.
Specimen Type: Bone marrow aspirate clot
Container/Tube: Bone marrow clot in 10% formalin or fixed clot embedded in paraffin block
1. Place 0.5 mL bone marrow aspirate in clot tube.
2. After clot has formed, place clot in 10% formalin.
3. If embedding in paraffin, fix specimen, process and embed per your standard operating procedures.
4. If submitting to Mayo for possible processing and embedding, submit in container of 10% formalin.
Specimen Type: Peripheral blood slides and CBC
Container/Tube: Transport in plastic slide holders.
Preferred: Fresh prep fingerstick slide; include CBC values
Acceptable: Slides made from whole blood in EDTA, made within 8 hours of collection
1. Prepare good quality smear of even thickness from fingerstick.
2. Alternately prepare good quality smear from EDTA vial within 8 hours of collection.
3. Stain with Wrights-Giemsa or submit unstained and unfixed slides.
Note: No specimen should be rejected. If specimen not received at appropriate temperature or in wrong anticoagulant, include note to laboratory. If questions, contact laboratory.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.