70016 - Specimen: Hematopathology Consultation, Wet Tissue

Test Catalog

Test Name

Test ID: 70016    
Hematopathology Consultation, Wet Tissue

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

A Mayo Clinic hematopathologist will provide a full bone marrow workup which includes an evaluation of the specimen and determination of a diagnosis provided within a formal pathology report.

When a consultation is requested, the client is responsible for submitting the following specimens:

1. Wet (fresh) core biopsy and/or bone marrow aspirate clot submitted in formalin

2. Bone marrow aspirate samples collected in:

a. Lavender top (EDTA): molecular testing

b. Yellow top (ACD): flow cytometric testing

c. Green top (sodium heparin): chromosome analysis and FISH testing

3. Slides prepared from bone marrow and peripheral blood

 

Ancillary Testing

Based on Mayo Clinic-approved algorithms and/or a staff hematopathologists' discretion, ancillary testing may be performed in order to render an accurate diagnosis and provide important prognostic information. These test results will be incorporated into the consultative report and will be billed and reported separately.

 

-Tier 1 Testing: eg, cytochemical stains on bone marrow aspirate smear, immunohistochemical stains on bone marrow biopsy or clot sections, chromosome analysis, FISH, flow cytometry and/or molecular testing will be added as appropriate. Results will be incorporated into the pathology report, as well as, some separate standalone results may be released. All will be charged.

-Tier 2 Testing: eg, Next Generation Sequencing (NGS) or microarray testing will only be added upon approval of the ordering physician/pathologist (client).

 

If ancillary testing (eg, flow cytometry) is desired by the client outside of this consultation, each test must be ordered separately. Tests ordered outside of the consultation may or may not be integrated into the final pathology report based on staff hematopathologist's discretion.

 

If the volume of bone marrow aspirate is limited, prioritization of testing will be determined by the staff hematopathologist. Testing requested/suggested by the referring physician (immunostains, molecular studies, etc) may not be performed if deemed unnecessary by the reviewing staff hematopathologist.

 

Note: Calls are not routinely made; however, depending on the nature of the case, a call may be placed to the ordering provider or pathologist. These situations include, but are not limited to, a new diagnosis of acute leukemia or aggressive high grade lymphoma. To contact a Mayo Clinic Hematopathologist, please call the Hematopathology Communications team, 507-284-5600.

 

If a client chooses to embed the clot/biopsy, the bone marrow aspirate specimens (tubes) should be sent in the same package as the bone marrow biopsy/clot sections. Embedding at the client site may delay specimen arrival at Mayo Medical Laboratories and preclude the ability to perform some ancillary testing due to specimen stability.

 

If a consultation is requested and only formalin-fixed, paraffin-embedded (FFPE) samples will be submitted, please order 70012 / Pathology Consultation.

Specimen Type Describes the specimen type needed for testing

Varies

Advisory Information

-If requesting a peripheral blood smear evaluation only, order SPSM / Morphology Evaluation (Special Smear).

-If requesting a Hematopathology consultation and only paraffin-embedded biopsy/clot samples (ie, no bone marrow aspirate) are submitted, order 70012 / Pathology Consultation.

Necessary Information

Necessary Information

All specimens, patient history, and requests must be clearly labeled with the correct patient information, case number, and specimen type.

 

In order to facilitate an effective work up the following information is required:

1. Hematopathology Patient Information (T676) in Special Instructions

2. Collection date

3. Patient date of birth

4. Patient's brief clinical history and reason for biopsy

5. Recent pertinent clinical notes

6. Most recent CBC

7. Pathology Report (if available)

8. All pending and finalized ancillary/pathology reports

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

See the following in Special Instructions for additional collection instructions:

-Assistance with Bone Marrow Collection

-Bone Marrow Core Biopsy, Clot, and Aspirate Collection Guideline

 

Specimen Type: Bone marrow core biopsy

Container/Tube: Fixed biopsy core in saline solution for transport or fixed biopsy core embedded in paraffin block

Collection Instructions:

1. Prepare 3 biopsy touch prep slides.

2. Place biopsy core in 10% formalin immediately after collection.

3. Fix in 10% formalin for transport.

4. Stretch parafilm around container for transport.

 

Specimen Type: Bone marrow aspirate clot

Container/Tube: Bone marrow clot in 10% formalin or fixed clot embedded in paraffin block

Collection Instructions:

1. Place 0.5 mL bone marrow aspirate in clot tube.

2. After clot has formed, place clot in 10% formalin solution for transport.

3. Do not combine with BM core biopsy.

4. Stretch parafilm around container for transport.

 

Specimen Type: Bone marrow aspirate in anticoagulant

Container/Tube: EDTA, heparin, or ACD

Specimen Volume: 3 mL in EDTA, 4 mL in ACD, or 3 mL in heparin tube

Collection Instructions:

1. Aspirate per standard bone marrow collection procedure.

2. For low volume collections, collect all in EDTA

3. Do not transfer to other tubes or containers.

 

Specimen Type: Bone marrow aspirate slides and CBC

Container/Tube: Transport in plastic slide holders.

Preferred: Fresh prep slides made at time of collection

Acceptable: Slides made from bone marrow aspirate in EDTA, made within 2 hours of collection

Collection Instructions:

1. Prepare slides of bone marrow aspirate immediately after collection.

2. Air dry slides.

3. Submit 5 (2 direct prep, 3 unit prep) unstained and unfixed slides.

4. Place slides in plastic slide holders. Slide carriers, must not have been previously used to carry fixed slides.

5. Stretch parafilm around slide carriers to prevent exposure to formalin fumes during transport.

6. Place slide carriers in a separate bag and apart from any formalin-fixed clot or core biopsy specimens during transport.

 

Specimen Type: Peripheral blood slides and CBC

Container/Tube: Transport in plastic slide holders.

Preferred: Fresh prep fingerstick slide; include CBC values

Acceptable: Slides made from whole blood in EDTA, made within 8 hours of collection

Collection Instructions:

1. Prepare good quality smear of even thickness from fingerstick.

2. Alternately prepare good quality smear from EDTA vial within 8 hours of collection.

3. Submit 2 unstained and unfixed slides.

4. Place slides in plastic slide holders. Slide carriers, must not have been previously used to carry fixed slides.

5. Stretch parafilm around slide carriers to prevent exposure to formalin fumes during transport.

6. Place slide carriers in a separate bag and apart from any formalin-fixed clot or core biopsy specimens during transport.

Forms

1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send Hematopathology/Cytogenetics Test Request Form (T726) (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Paraffin-embedded biopsy/clot samples without bone marrow aspirate. Call the client and change the order to 70012 / Pathology Consultation.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)
 Refrigerated