70015 - Specimen: Anatomic Pathology Consultation, Wet Tissue

Test Catalog

Take Our Survey

Test ID: 70015    
Anatomic Pathology Consultation, Wet Tissue

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

An interpretation will be provided by Mayo staff pathologists within a formal pathology report which is sent to the referring pathologist.


-This test should be used when submitting lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea and other small eye biopsies for Mayo pathology consultation/interpretation.

-Appropriate stains and other ancillary testing may be performed and charged separately.

-If requesting a pathology consultation on paraffin embedded tissue and slides only (ie, no wet tissue being sent to be processed), order 70012 / Pathology Consultation instead.


Note: Tests requested by the referring physician (eg, immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo pathologist.

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

All specimens, patient history, and requests should be clearly labeled with correct patient identifiers and pathology accession/case number. Collection date and patient date of birth are required. All specimens must be labeled with specimen type. Although a complete pathology report is not expected to be submitted with the specimen, a preliminary pathology report is required in order to ensure that the specimen is known to a referring pathologist. The name and phone number of the ordering pathologist and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.


Specimen Type: Lung biopsy

Container/Tube: Pathology Packaging Kit (Supply T554)

Collection Instructions:

1. Submit portion of lung tissue in formalin for light microscopy processing.

2. Place a portion of the lung tissue in Zeus preservative for immunofluorescence.

3. A representative H and E slide from the light microscopy sample must be submitted along with the tissue in Zeus preservative.

Additional Information: Immunofluorescence on lung tissue must be ordered in conjunction with 70015 / Anatomic Pathology Consultation, Wet Tissue.


Specimen Type: Cardiac biopsy

Container/Tube: Pathology Packaging Kit (Supply T554)

Collection Instructions:

1. For ideal analysis, collect up to 4 to 6 biopsy specimens and submit all in formalin unless electron microscopy (EM) is requested.

2. If EM is needed, submit at least one biopsy into 2.5% to 3% buffered glutaraldehyde preservative using the Electron Microscopy Kit (Supply T660), or any leak-proof container such as a 50 mL conical vial (Supply T080). See also Electron Microscopy Procedures for Handling Specimens for Electron Microscopy (Test #4993) in Special Instructions

Additional Information: If the question is drug toxicity, storage disease, or if the patient is <25 years old, send at least 1 biopsy specimen in 2.5% to 3% buffered glutaraldehyde in case electron microscopy (EM) is needed for diagnosis.

Forms: If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the cardiac biopsy specimen (http://www.mayomedicallaboratories.com/it-mmfiles/cardiovascular-request-form.pdf).


Specimen Type: Cardiac explant

Container/Tube: Pathology Packaging Kit (Supply T554)

Collection Instructions:

1. Fix entire explant specimen in formalin for a minimum of 24 hours.

2. Following fixation, lightly wrap specimen in formalin-soaked paper towel, place in a sealable bag with a small amount of formalin, seal, and ship ambient.

Additional Information: Please include the last pre-transplantation echocardiogram with report and images as well as all other relevant clinical documents,


Specimen Type: Enucleated eye

Container/Tube: Pathology Packaging Kit (Supply T554)

Specimen Volume: Entire specimen

Collection Instructions:

1. Immediately after removal, place specimen in approximately 300 mL of 10% formalin.

2.  Enucleated eye should not be opened or punctured. After 48 hours in the originating laboratory, the specimen should be transferred to a smaller container (eg, 50 mL conical vial-Supply T080) with fixative (10% formalin) and sealed to be shipped without risk of leakage.


Specimen Type: Iris, conjunctiva, cornea, and other small biopsies from eye

Container/Tube: Pathology Packaging Kit (Supply T554)

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain biopsy.

2. Place iris, conjunctiva, cornea and other small biopsies on a piece of filter paper or other porous paper (such as that which wraps rubber gloves.) This helps to keep the specimen from curling when it is fixed.

3. Immediately but gently place specimen (on the paper mount) into 10% formalin, approximately 20 times the volume of the biopsy. Place in suitable leak-proof container such as 50 mL conical vial (Supply T080).

4. Placing sutures at the margins of resection with accompanying explanatory draft will help orientation of the specimen in the lab, in cases where margins are important.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.


Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected










Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)