70015 - Specimen: Anatomic Pathology Consultation, Wet Tissue

Test Catalog

Test ID: 70015    
Anatomic Pathology Consultation, Wet Tissue

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

An interpretation will be provided by Mayo staff pathologists within a formal pathology report that is sent to the referring pathologist.

 

-This test should be used when submitting lung tissue for immunofluorescence, cardiac biopsies, enucleated eye specimens, iris, conjunctiva, cornea and other small eye biopsies for Mayo pathology consultation/interpretation.

-Appropriate stains and other ancillary testing may be performed and charged separately.

-If requesting a pathology consultation on paraffin embedded tissue and slides only (ie, no wet tissue being sent to be processed), order 70012 / Pathology Consultation instead.

 

Note: Tests requested by the referring physician (eg, immunostains, molecular studies, etc.) may not be performed if they are deemed to be unnecessary by the reviewing Mayo Clinic pathologist. For all pathology consultations, ancillary testing is ordered at the discretion of the Mayo pathologist.

Specimen Type Describes the specimen type needed for testing

Varies

Additional Testing Requirements

Lung biopsy specimens: Immunofluorescence on lung tissue must be ordered in conjunction with this test.

 

Vitreous fluid/Aqueous humor specimens: Call Mayo Medical Laboratories (800-533-1710) to request an Expedite (cost=$25) on this order since vitreous fluid will have no fixative and needs to reach the laboratory ASAP.

Shipping Instructions

Vitreous fluid/Aqueous humor specimens: ship overnight Attention: Ophthalmic Pathology Working Group

Necessary Information

All specimens, patient history, and requests should be clearly labeled with correct patient identifiers and pathology accession/case number.

 

Collection date and patient date of birth are required. All specimens must be labeled with specimen type.

 

Although a complete pathology report is not expected to be submitted with the specimen, a preliminary pathology report is required in order to ensure that the specimen is known to a referring pathologist. The name and phone number of the ordering pathologist and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Supplies: Pathology Packaging Kit (T554)

Specimen Type: Lung biopsy

Collection Instructions:

1. Submit portion of lung tissue in formalin for light microscopy processing.

2. Place a portion of the lung tissue in Zeus preservative for immunofluorescence.

3. A representative H and E slide from the light microscopy sample must be submitted along with the tissue in Zeus preservative.

 

Supplies:

Pathology Packaging Kit (T554)

Electron Microscopy Kit (T660)

Conical Tube, 50 mL (T080)

Specimen Type: Cardiac biopsy

Collection Instructions:

1. For ideal analysis, collect 4 to 6 biopsy specimens and submit all in formalin unless electron microscopy (EM) is requested.

2. If EM is needed, submit at least 1 biopsy into 2.5% to 3% buffered glutaraldehyde preservative using the Electron Microscopy Kit (T660), or any leak-proof container such as a 50-mL conical tube (T080). See also Electron Microscopy Procedures for Handling Specimens for Electron Microscopy in Special Instructions.

Additional Information: If the question is drug toxicity, storage disease, or if the patient is <25 years old, send at least 1 biopsy specimen in 2.5% to 3% buffered glutaraldehyde in case electron microscopy (EM) is needed for diagnosis.

 

Supplies: Pathology Packaging Kit (T554)

Specimen Type: Cardiac explant

Collection Instructions:

1. Fix entire explant specimen in formalin for a minimum of 24 hours.

2. Following fixation, lightly wrap specimen in formalin-soaked paper towel, place in a sealable bag with a small amount of formalin, seal, and ship ambient.

Additional Information: Please include the last pretransplantation echocardiogram with report and images as well as all other relevant clinical documents.

 

Supplies:

Pathology Packaging Kit (T554)

Conical Tube, 50 mL (T080)

Specimen Type: Enucleated eye

Specimen Volume: Entire specimen

Collection Instructions:

1. Immediately after removal, place specimen in approximately 300 mL of 10% formalin.

2. Enucleated eye should not be opened or punctured. After 48 hours in the originating laboratory, the specimen should be transferred to a smaller container (eg, 50-mL conical tube: T080) with fixative (10% formalin) and sealed to be shipped without risk of leakage.

 

Supplies:

Pathology Packaging Kit (T554)

Conical Tube, 50 mL (T080)

Specimen Type: Iris, conjunctiva, cornea, and other small biopsies from eye

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain biopsy.

2. Place iris, conjunctiva, cornea, and other small biopsies on a paper mount (a piece of filter paper or other porous paper). This helps to keep the specimen from curling when it is fixed.

3. Immediately but gently place specimen (on the paper mount) into 10% formalin, approximately 20 times the volume of the biopsy. Place in suitable leak-proof container such as 50-mL conical tube (T080).

4. Placing sutures at the margins of resection with accompanying explanatory draft will help orientation of the specimen in the lab, in cases where margins are important.

 

Supplies:

Pathology Packaging Kit (T554)

Aliquot Tube, 5 mL (T465)

Specimen Type: Vitreous fluid/Aqueous humor

Specimen Volume: Entire specimen

Collection Instructions:

1. Obtain liquid specimen.

2. Place the liquid (vitreous or aqueous) concentrate into a small tube with a screw-top cap (T465). Seal the tube tightly.

3. Put the specimen immediately on a refrigerated cool pack (not dry ice or frozen cool pack).

4. If a washing (diluted) is available, it can also be sent in similar manner.

Forms

Cardiac Biopsy: If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the cardiac biopsy specimen (http://www.mayomedicallaboratories.com/it-mmfiles/cardiovascular-request-form.pdf).

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Varies

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

 

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)
 Refrigerated