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Test Catalog

Test ID: MPRP    
Mycoplasma pneumoniae, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma pneumoniae DNA is not likely.


Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (

Neurology Test Request Form (T732) (


Specimen source is required.


Submit only 1 of the following specimens:


Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum


Preferred: Sterile container

Acceptable: Place specimen in M4, M5, or UTM

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated 7 days


Specimen Type: Swab

Sources: Throat or nasopharyngeal


Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M5, UTM, or  ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

Specimen Stability Information: Refrigerated 7 days/Frozen 7 days


Specimen Type: Fluid

Sources: Pleural/chest, pericardial, cerebrospinal

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Nonswab specimen: 0.5 mL; Swab: 1 swab

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesVaries7 days