Mycoplasma pneumoniae, Molecular Detection, PCR
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma pneumoniae DNA is not likely.
Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Microbiology Request Form (T244) (www.mayomedicallaboratories.com/media/customer-service/forms/microbiology-request-form.pdf)
Neurology Test Request Form (T732) (http://www.mayomedicallaboratories.com/media/customer-service/forms/neurology-request-form.pdf)
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
Preferred: Sterile container
Acceptable: Place specimen in M4, M5, or UTM
Specimen Volume: 1 mL
Specimen Stability Information: Refrigerated 7 days
Specimen Type: Swab
Sources: Throat or nasopharyngeal
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M5, UTM, or ESwab
Specimen Volume: Swab
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Stability Information: Refrigerated 7 days/Frozen 7 days
Specimen Type: Fluid
Sources: Pleural, pericardial, cerebrospinal
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Nonswab specimen: 0.5 mL; Swab: 1 swab
Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.