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Test ID: HTGFN    
Thyroglobulin, Tumor Marker, Fine-Needle Aspiration (FNA)-Needle Wash, Lymph Node

Specimen Type Describes the specimen type needed for testing

Fine Needle Wash

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Plain, plastic, screw-top tube

Collection Instructions: After collection of the cytology specimens and expulsion of the material for smear or CytoTrap processing:

1. Wash/rinse each FNA needle from a single lymph node nodule with 0.1 to 0.5 mL of normal saline.

2. Pool each wash from a single lymph node nodule into 1 tube. If more than 1 nodule is biopsied, each nodule biopsy should be submitted as a separate specimen.

3. Inspect the specimen as follows:

a. If the specimen shows visible blood or tissue contamination, centrifuge the specimen. Transfer the supernatant to a new plastic tube. Freeze specimen.

b. If specimen is clear, freeze specimen in plastic tube.

Additional Information: If multiple lymph node testing is needed, submit each under a separate order. Clearly identify each specimen.

Forms: If not ordering electronically, submit a General Request Form (Supply T239) with the specimen.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL (Specimens <0.5 may be rejected)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject







Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Fine Needle WashFrozen (preferred)7 days
 Refrigerated 7 days