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Test ID: CATLN    
Calcitonin, Fine-Needle Aspiration Biopsy (FNAB)-Needle Wash, Lymph Node

Specimen Type Describes the specimen type needed for testing

Fine Needle Wash

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Plain, plastic, screw-top tube

Specimen Volume: 1.5 mL

Collection Instructions: Needle wash specimens for analysis should be collected in conjunction with cytology specimens.

1. Have saline available prior to start of procedure. Saline is the only acceptable solution for needle washings.

2. After each fine-needle aspiration biopsy (FNAB) has been collected and the material in the needle has been expelled onto a slide for cytologic analysis, attach the used FNAB needle to an empty syringe.

3. Withdraw between 0.10-0.25 mL of saline up through the needle until the saline starts to fill the hub of the needle or end of the syringe.

4. Expel this fluid back through the needle into a separate tube. This is the needle washing used for analysis.

5. Repeat steps 2 through 4 for each needle pass of the same biopsied site and empty into the same tube, accumulating a total of 0.5-1.5 mL of fluid to send to the laboratory. (If more than 1 site is biopsied, see Note)

6. Inspect specimen for visible blood or tissue contamination:

 -a. If bloody, centrifuge specimen and transfer supernatant to a new plastic aliquot tube (5-mL standard tube) to send to laboratory. The supernatant, not the cellular material, is used for analysis.

 -b. If specimen is clear, centrifugation is not necessary.

7. Refrigerate within 1 to 2 hours of collection and freeze within 2 to 4 hours of collection. Send frozen to the laboratory.

Note: If more than 1 site is biopsied, each washing material should be submitted on a separate tube and under a different order number. The biopsied site of each specimen must be clearly identified in LIS and/or batch sheet.

Additional Information: A minimum of 0.5 mL is required for testing; however, the total collection volume should not exceed 1.5 mL. Sample volumes outside these parameters may be rejected.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Fine Needle WashFrozen (preferred)7 days
 Refrigerated 4 hours