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Test ID: PTHFN    
Parathyroid Hormone, Fine-Needle Aspiration Biopsy (FNAB)-Needle Wash

Specimen Type Describes the specimen type needed for testing

Fine Needle Wash

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Plain, plastic, screw-top tube

Specimen Volume: 1-1.5 mL

Collection Instructions: After collection of the cytology specimens and expulsion of the material for smear or CytoTrap process as follows:

1. Wash/rinse each FNA needle from a single biopsied area with 0.1 to 0.5 mL of normal saline.

2. Pool each wash from a single biopsied area into 1 tube.

3. If more than 1 area is biopsied, each biopsy area should be submitted as a separate specimen under a separate order. Clearly identify each specimen.

4. Inspect the specimen as follows:

a. If the specimen shows visible blood or tissue contamination, centrifuge the specimen. Transfer the supernatant to a new plastic tube. Freeze specimen.

b. If specimen is clear, freeze specimen in plastic tube.

c. Specimen should be frozen within 4 hours of collection.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL (Samples <0.5 mL may be rejected)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


 Mild reject; Gross reject




 Mild OK; Gross reject



Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Fine Needle WashFrozen (preferred)30 days
 Refrigerated 4 hours