TPPA - Specimen: Syphilis Antibody by TP-PA, Serum

Test Catalog

Test ID: TPPA    
Syphilis Antibody by TP-PA, Serum

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

See Syphilis Serology Algorithm in Special Instructions.

Specimen Type Describes the specimen type needed for testing


Advisory Information

This assay is recommended by the CDC for specimens testing positive by a screening treponemal assay and negative by RPR. The results of this assay assist in determining whether the results of a screening treponemal test are truly or falsely positive.


This test is not intended for medical-legal use.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.3 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject


Mild OK; Gross reject





Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
SerumRefrigerated (preferred)14 days
 Frozen 14 days