IDH1/IDH2 Mutation Analysis by Pyrosequencing, Paraffin
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
A pathology or diagnostic report, including a brief history, is required. If available, include IDH1 mutation (R132H) immunostain results.
Preferred: Formalin-fixed, paraffin-embedded (FFPE) tissue block with a minimum of 40% tumor cell population
Alternate: Unstained slides with a minimum of 40% tumor population; slides may be stained and/or scraped
1. Process all fresh or frozen specimens into FFPE blocks prior to submission.
2. If submitting slides, a minimum of five, 4- to 5-micron thick, unstained slides are required.
1. A quality specimen is essential for evaluation. Submit only tissue containing tumor cells; minimal tissue is required for evaluation.
2. Special stains performed outside Mayo Medical Laboratories and included with the case may be repeated and charged at the reviewing pathologist's discretion. Testing requested by referring physician may not be performed if deemed unnecessary by Mayo Clinic pathologist.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.