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Test Catalog

Test ID: MHRP    
Mycoplasma hominis, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma hominis DNA is not likely.


Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (


Specimen source is required.


Submit only 1 of the following specimens:


Specimen Type: Swab

Sources: Cervix, urethra, urogenital, vaginal, wound; bronchus (donor swab); upper respiratory sources (only infants <3 months: nasopharynx, nose, throat)


Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Swab in transport media: M4, M5 (Supply T484), universal transport media, or ESwab

Specimen Volume: 1 swab

Collection Instructions:


1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

Urethra or Cervical:

1. Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

2. Place swab back into swab cylinder.


1. Collect specimen by swabbing back and forth over wound surface to maximize recovery of cells.

2. Place swab back into swab cylinder.


Specimen Type: Fluid

Sources: Pelvic fluid, amniotic fluid, prostatic secretions, semen, reproductive drainage or fluid, synovial fluid, pleural/chest fluid, pericardial fluid, sputum, tracheal secretions, bronchial washings, bronchoalveolar lavage, lung


Preferred: Sterile container

Acceptable: Container with 3 mL of transport media: M4, M5 (Supply T484), or universal transport media

Specimen Volume: 1-2 mL


Specimen Type: Urine, kidney stone, ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen


Specimen Type: Tissue

Sources: Placenta, products of conception, genitourinary, respiratory, bronchus, chest/mediastinal, wound

Container/Tube: Sterile container

Specimen Volume: 5 mm

Collection Instructions: Submit only fresh tissue.


Specimen Type: EDTA plasma

Container/Tube: Sterile vial

Specimen Volume: 1 mL


Specimen Type:  Whole blood 


Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA aliquot

Specimen Volume: 1 mL

Collection Instructions: Send specimen in original tube.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Fluid: 1 mL; Plasma, Whole Blood: 1 mL; Urine: 2 mL; Swab: 1 swab

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal, formalin-fixed and/or paraffin-embedded tissues, Port-a-Cul tube, anaerobic fluid vials, or dry swab (no pledget or sponge)

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Frozen 7 days