Test ID: SEQF
Sequential Maternal Screening, Part 2, Serum
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Sequential maternal screening is a 2-part test that includes a first-trimester sample (SEQU/60698 Sequential Maternal Screening, Part 1, Serum) and a second-trimester sample (SEQF/60700 Sequential Maternal Screening, Part 2, Serum). Do not order this test unless you have already ordered test SEQU/60698 Sequential Maternal Screening, Part 1, Serum. If a stand alone second-trimester screen is desired, order QUAD/81149 Quad Screen (Second Trimester) Maternal, Serum.
Draw blood between 15 weeks, 0 days and 22 weeks, 6 days. Do not draw blood after performing amniocentesis, as that may lead to an artificially increased serum alpha-fetoprotein (AFP) level and unreliable results.
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 1 mL
Collection Instructions: Spin down immediately.
Additional Information: Draw date is required.
Forms: Second Trimester Maternal Screening Alpha-Fetoprotein (AFP)/Quad Screen Patient Information Sheet in Special Instructions
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross OK |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 90 days |
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