LPMAF - Specimen: Lymphocyte Proliferation Panel for Mitogens and Antigens

Test Catalog

Test ID: LPMAF    
Lymphocyte Proliferation Panel for Mitogens and Antigens

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test code will be reflexed and billed separately.

Specimen Type Describes the specimen type needed for testing

WB Sodium Heparin

Advisory Information

LPAGF / Lymphocyte Proliferation to Antigens, Blood testing should not be ordered for patients less than 3 months of age unless there is a clinical history of Candidiasis. See the Cautions section for additional information. Order test LPMGF / Lymphocyte Proliferation to Mitogens, Blood instead of this test for patients less than 3 months of age, as appropriate for clinical history.

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668).

Necessary Information

Date and time of draw and ordering physician's name and phone number are required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

For serial monitoring, we recommend that specimen draws be performed at the same time of day.


Container/Tube: Green top (sodium heparin)

Specimen Volume:

See Cautions section.

<3 months: 3 mL

3-24 months: 5 mL

25 months-5 years: 6 mL

6-18 years: 8 mL

>18 years: 30 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

<3 months: 1 mL; 3-24 months: 3 mL; 25 months-18 years: 5 mL; Adults (>18 years): 20 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject


Mild OK; Gross reject




Lithium heparin


Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
WB Sodium HeparinAmbient48 hours
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