Lymphocyte Proliferation to Antigens, Blood
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test code will be reflexed and billed separately.
Specimen Type Describes the specimen type needed for testing
WB Sodium Heparin
If both antigens and mitogens are desired, order LPMAF / Lymphocyte Proliferation Panel for Mitogens and Antigens.
Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668).
1. Date and time of draw and ordering physician name and phone number are required.
2. Specify "Antigen" to differentiate from "Mitogen" testing. This information is required.
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
This test should not be ordered for patients <3 months of age unless there is a clinical history of Candidiasis. See the Cautions section for additional information.
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Container/Tube: Green top (sodium heparin)
See Cautions section.
<3 months: 1 mL
3-24 months: 3 mL
25 months-18 years: 5 mL
>18 years: 20 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
<6 years: 1 mL; 6-18 years: 2 mL; Adults (>18 years): 6 mL
Mild OK; Gross reject
Mild OK; Gross reject
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|WB Sodium Heparin||Ambient||48 hours|