LPMGF - Specimen: Lymphocyte Proliferation to Mitogens, Blood

Test Catalog

Test Name

Test ID: LPMGF    
Lymphocyte Proliferation to Mitogens, Blood

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test code will be reflexed and billed separately.

Specimen Type Describes the specimen type needed for testing

WB Sodium Heparin

Advisory Information

 

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw.

Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668).

Specimens arriving on the weekend may be canceled.

Necessary Information

1. Date and time of draw and ordering physician name and phone number are required.

2. Specify "Mitogen" to differentiate from "Antigen" testing. This information is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Supplies: Ambient Mailer-Critical Specimens Only (T668)

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3 months-5 years: 2 mL

6-18 years: 3 mL

>18 years: 10 mL

Collection Instructions: Send specimen is original tube. Do not aliquot.

 

Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)

 

Mitogen Only

ALC

Blood Volume for Minimum PHA Only

Blood Volume for Minimum PHA and PWM

Blood Volume for Full Assay

<0.5

>6.5 cc

>8.5 cc

>22 cc

0.5-1.0

6.5 cc

8.5 cc

22 cc

1.1-1.5

3.0 cc

4.0 cc

10 cc

1.6-2.0

2.0 cc

2.5 cc

7 cc

2.1-3.0

1.5 cc

2.0 cc

6 cc

3.1-4.0

1.0 cc

1.5 cc

4 cc

4.1-5.0

0.8 cc

1.0 cc

3 cc

>5.0

0.5 cc

0.8 cc

2 cc

 

Mitogen and Antigen

ALC

Blood Volume for Minimum of Each Assay

Blood Volume for Full Assay

<0.5

>28 cc

>60 cc

0.5-1.0

28 cc

60 cc

1.1-1.5

12 cc

30 cc

1.6-2.0

8.5 cc

20 cc

2.1-3.0

6.5 cc

15 cc

3.1-4.0

4.5 cc

10 cc

4.1-5.0

3.5 cc

8 cc

>5.0

2.5 cc

6 cc

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

Lithium heparin; Green top microtube (lithium heparin); Aliquot tube

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
WB Sodium HeparinAmbient48 hours