TCIPF - Specimen: T- and B-Cell Quantitation and Lymphocyte Proliferation to Antigens and Mitogens Panel

Test Catalog

Test ID: TCIPF    
T- and B-Cell Quantitation and Lymphocyte Proliferation to Antigens and Mitogens Panel

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

If insufficient peripheral blood mononuclear cells (PBMCs) are isolated from the patient's sample due to low WBC counts or specimen volume received, selected dilutions or stimulants may not be tested at the discretion of the laboratory to ensure the most reliable results. Testing with 1 stimulant will always be performed. When adequate specimen is available for both stimulants to be tested, an additional test code will be reflexed and billed separately.

 

When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received and the laboratory will cancel those for which the appropriate specimen was not received. Be advised that this may change the degree of interpretation received with the report.

Specimen Type Describes the specimen type needed for testing

WB Sodium Heparin
Whole Blood EDTA

Advisory Information

Lymphocyte Proliferation to Antigens, Blood (LPAGF) testing should not be ordered for patients less than 3 months of age unless there is a clinical history of Candidiasis. See the Cautions section for additional information. Order test TCMPF / T, B, and NK Lymphocyte Quantitation and Lymphocyte Proliferation to Mitogens Panel instead of this test for patients less than 3 months of age, as appropriate for clinical history.

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668).

Necessary Information

Date and time of draw and ordering physician's name and phone number are required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Three separate specimens are required.

 

Specimen Type: EDTA whole blood

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

 

Specimen Type: Sodium heparin whole blood

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3-24 months: 3 mL

25 months-18 years: 5 mL

>18 years: 20 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

 

Specimen Type: Sodium heparin whole blood

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3 months-5 years: 2 mL

6-18 years: 3 mL

>18 years: 10 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

EDTA whole blood: 1 mL; Sodium heparin whole blood <6 years: 1 mL/6-18 years: 2 mL/Adults (>18 years): 6 mL; Sodium heparin whole blood: 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

Specimens in aliquot tubes (not in original Vacutainer)

 

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
WB Sodium HeparinAmbient48 hours
Whole Blood EDTAAmbient52 hours