ENFD - Specimen: Epidermal Nerve Fiber Density Consult

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Test ID: ENFD    
Epidermal Nerve Fiber Density Consult

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, 1 or more of the listed reflex tests will be performed and charged for each biopsied site.

 

Wet tissue for consultation: When adequately prepared tissue is provided, routine testing will include: PGP 9.5 immunostain, morphometric analysis, Congo red stain, and hematoxylin and eosin stain.

 

Slides and blocks sent for consultation must include PGP 9.5 reacted sections:

Special stains and studies performed on the case should be sent with the case for review. In order to determine an accurate diagnosis, some of these stains or studies may be deemed to warrant repeat testing at an additional charge at the discretion of the reviewing Mayo Clinic neuromuscular pathologist. In addition, testing requested by the referring physician (immunostains, molecular studies, etc) may not be performed if deemed unnecessary by the reviewing Mayo Clinic neuromuscular pathologist. For all consultations, ancillary testing necessary to determine a diagnosis is ordered at the discretion of the Mayo Clinic neuromuscular pathologist. An interpretation, which includes an evaluation of the specimen and determination of a diagnosis, will be provided within a formal pathology report.

Specimen Type Describes the specimen type needed for testing

Tissue

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Forms:

1. Epidermal Nerve Fiber Density Patient Information (T702) in Special Instructions

2. Epidermal Nerve Fiber Density Instructions (T703) in Special Instructions

 

Preferred:

Specimen Type: Skin punch biopsy tissue

Collection Instructions:

1. A Skin Punch Biopsy Kit containing fixatives, buffer, and cryoprotectant is required (no substitutions accepted). For ordering information, call 507-284-8065.

2. Prepare and transport specimen per instructions on the Epidermal Nerve Fiber Density Instructions (T703) in Special Instructions.

Additional Information: Neurology Clinical Notes information is required on Epidermal Nerve Fiber Density Patient Information (T702) in Special Instructions.

Specimen Stability: Refrigerated (preferred)/Ambient

 

Acceptable:

Specimen Type: Slides

Additional Information:

1. Slides reacted with PGP 9.5 are required.

2. Hematoxylin and eosin-stained slides and Congo red-stained slides are optional.

Specimen Stability: Ambient (preferred)/Refrigerated

 

Specimen Type: Tissue block and PGP9.5-reacted slides

Collection Instructions:

1. Slides reacted with PGP 9.5 are required.

2. Tissue block may be used to create hematoxylin and eosin-stained slides and Congo red-stained slides.

Specimen Stability: Ambient (preferred)/Refrigerated

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

NA

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Note: No specimen should be rejected. If questions, contact laboratory.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
TissueVaries

Key