HV1CM - Specimen: HIV-1 and HIV-2 Antibody Screen for Hemolyzed Specimens, Serum

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Test ID: HV1CM    
HIV-1 and HIV-2 Antibody Screen for Hemolyzed Specimens, Serum

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

This test begins with HIV-1/-2 antibody EIA. If HIV-1/-2 antibody EIA is reactive, then HIV antibody confirmation/differentiation (by immunochromatographic method) is performed at an additional charge.

 

This is the appropriate test for testing asymptomatic individuals.

 

This test is not appropriate for testing symptomatic individuals. For testing hemolyzed specimens from symptomatic patients (ie, diagnostic purposes) with or without risk factors for HIV infection, order HV1CD / HIV-1 and HIV-2 Antibodies for Cadaveric or Hemolyzed Specimens, Serum.

 

See HIV Testing Algorithm (Fourth-Generation Screening Assay) Including Follow-up of Reactive HIV Rapid Serologic Test Results in Special Instructions.

Specimen Type Describes the specimen type needed for testing

Serum

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Spin down and remove serum from clot within 24 hours.

Additional Information: If the initial EIA result is negative and this test was ordered as a follow-up evaluation of a specimen with a reactive rapid HIV antibody test result, clients must call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700 to request supplemental testing for HIV antibody confirmation/differentiation by immunochromatography (HIVDI). The HIVDI / HIV-1 and HIV-2 Antibody Confirmation and Differentiation, Serum test is not FDA-approved for testing cadaveric specimens. If performed, test results will be reported with a disclaimer.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
SerumFrozen (preferred)30 days
 Ambient 7 days
 Refrigerated 7 days

Key