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Test ID: HBRP    
Histoplasma capsulatum/Blastomyces dermatitidis, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Histoplasma or Blastomyces species DNA is not likely.

 

Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.

 

 Preferred Specimens:  Body fluid, bone marrow, cerebrospinal fluid (CSF), respiratory (eg, bronchoalveolar lavage: BAL, bronchial washing, sputum), tissue (fresh), or bone

 

Acceptable Specimens: If no fresh specimen is available, digested respiratory specimens treated with N-acetyl-L-cysteine (NALC)/NaOH are acceptable (eg, BAL, bronchial washing, respiratory fluid, sputum, or tracheal secretion), as are NALC/NaOH-treated gastric washings.

 

Unacceptable Specimens: Specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), stool, swab, tissue in formalin fluid, urine, or specimen >7 days old

 

Specimen must arrive within 7 days of collection; specimen >7 days will be rejected.

 

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Body, CSF, bone marrow

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

 

Specimen Type: Respiratory

Sources: BAL, bronchial washing, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

 

Specimen Type: Tissue or bone

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions: Collect a fresh tissue or bone specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

 

Acceptable:

NALC/NaOH-digested specimen must arrive within 7 days of digestion.

                                                               

Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Body Fluid or Respiratory Specimen: 0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), stool, swab, tissue in formalin fluid, urine, specimen >7days old

 

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Ambient 7 days
 Frozen 7 days