HER2, Gastric/Esophageal, Semi-Quantitative Immunohistochemistry, Manual
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
If being ordered for diagnostic purposes, you must order a surgical pathology consultation test (5439) and then request the stain.
If being ordered for prognostic purposes:
Preferred: Paraffin-embedded tissue block containing invasive gastric or esophageal adenocarcinoma tissue that has been fixed in 10% neutral buffered formalin shipped at ambient temperature
Acceptable: 2 unstained sections, containing gastric or esophageal adenocarcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature. Tissue on the slides should be fixed in 10% neutral buffered formalin.
Container/Tube: Surgical Pathology Packaging Kit (Supply T554)
1. Submit paraffin-embedded gastric or esophageal adenocarcinoma tissue.
2. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable only if it refers to invasive or metastatic gastric or esophageal adenocarcinoma.
3. Attach the green pathology address label included in the kit to the outside of the transport container.
1. Only paraffin-embedded (FFPE) gastric or esophageal adenocarcinoma specimens will be accepted. Fixation in 10% neutral buffered formalin is preferred. For breast cancer specimens, order 81504 HER2, Breast, Quantitative Immunohistochemistry, Automated.
2. The performance and quality of immunohistochemical stains in 10% neutral buffered FFPE tissue depends on proper fixation. It is recommended (not required) for surgical specimens to be fixed between 18 and 24 hours and biopsy specimens between 6 and 8 hours.
3. Paraffin blocks will be returned with final report.
Forms: If not ordering electronically, submit a Pathology/Cytology Request Form (Supply T246) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.