MMLSA - Specimen: Antimicrobial Susceptibility, Anaerobic Bacteria, MIC

Test Catalog

Test ID: MMLSA    
Antimicrobial Susceptibility, Anaerobic Bacteria, MIC

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, Anaerobe Suscep Battery will be performed at an additional charge. All bacterial organisms submitted will automatically have susceptibility testing performed and billed as appropriate. Antimicrobial agents appropriate to the organism and specimen source will be chosen from the panels listed below.


The following antimicrobials are routinely tested for the various groups of anaerobes: 

Anaerobic gram-negative bacilli

Beta-lactamase positive

Piperacillin/tazobactam, ertapenem, metronidazole, clindamycin

Anaerobic gram-negative bacilli

Beta-lactamase negative

Penicillin, clindamycin, metronidazole

Anaerobic cocci and nonspore-forming gram-positive bacilli, Clostridium perfringens

Penicillin, clindamycin, metronidazole

Clostridium species other than Clostridium perfringens

Penicillin, clindamycin, metronidazole, ertapenem, piperacillin/tazobactam

Propionibacterium species

Penicillin, moxifloxacin, minocycline

Actinomyces species

Penicillin, clindamycin

For antimicrobial agents not part of above panels: testing is available upon special request at an additional charge per antimicrobial: Amoxicillin/clavulanic acid, ceftriaxone, meropenem, ciprofloxacin, and vancomycin.

Note: Amoxicillin/clavulanic acid is only prepared Monday through Friday.


If fewer than 3 antibiotics will be reported, then Anaerobe Suscep Battery will be canceled and Anaerobe Suscep per agent will be charged per antibiotic.


Based on susceptibility criteria, Beta Lactamase and/or Anaerobe Suscep per agent may be performed at an additional charge.

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Sources: Available on isolates from blood cultures, bone and joint infections, or brain abscesses and organisms isolated in pure culture from other sources


Preferred: Anaerobic transport tube (Supply T588)

Acceptable: Thioglycollate broth or any other suitable anaerobic transport system

Specimen Volume: Organism in pure culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (Supply T146) and label as an etiologic agent/infectious substance.

Additional Information:

1. Organism identification and specimen source are required for processing.

2. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.


Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Agar plate

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime