Test ID: 5434
Hematopathology Consultation
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: If not ordering electronically, please submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.
Consultation
Specimen Type: Peripheral blood, bone marrow aspirate, hematoxylin-and-eosin-stained slides of bone marrow biopsy, paraffin blocks
Container/Tube: Wright-Giemsa-stained slides
Collection Instructions:
1. Send stained slides of peripheral blood with CBC/differential.
2. When appropriate, include special stains and/or unstained slides.
3. Label with specimen type.
Additional Information:
1. Collection date is required.
2. A pathology/diagnostic report, the name and phone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician's needs.
Enzyme Cytochemical Stains
Specimen Type: Bone marrow aspirate
Container/Tube: Plastic slide container
Specimen Volume: 2 or more smears
Collection Instructions:
1. All smears for cytochemical stains should be air-dried, unfixed, unstained, and no more than 2 weeks old.
2. Label specimen as bone marrow aspirate.
Additional Information: Cytochemical stains will be performed only if diagnostically indicated.
Immunohistochemical Stains
Specimen Type: Tissue
Container/Tube: Paraffin blocks or appropriate number of unstained tissue sections on charged slides
Collection Instructions: Label specimen as tissue.
Additional Information: Immunohistochemical stains will be performed only if diagnostically indicated.
Leukemia Immunophenotyping by Flow Cytometry
Specimen Type: Peripheral blood
Container/Tube: Green top (heparin) or yellow top (ACD solution B)
Specimen Volume: 10 mL
Collection Instructions:
1. Do not transfer blood to other containers.
2. Include 5 to 10 unstained peripheral blood smears, if possible.
3. Label specimen as blood.
Additional Information: Immunophenotyping will be performed only if diagnostically indicated.
Specimen Type: Bone marrow
Container/Tube: Yellow top (ACD solution B)
Specimen Volume: 1-5 mL
Collection Instructions:
1. Include 2 to 5 unstained bone marrow aspirate smears, if possible.
2. Label specimen as bone marrow.
Additional Information: Immunophenotyping will be performed only if diagnostically indicated.
Specimen Type: Tissue (eg, lymph nodes) other than blood and bone marrow
Container/Tube: Sterile container with tissue culture medium (eg, Hank's balanced salt solution [Supply T132], RPMI, or equivalent)
Specimen Volume: 1 cm(3) biopsy
Collection Instructions: Label specimen as tissue.
Additional Information: If, in the opinion of the Mayo hematopathology consultant, the tissue is not adequate for flow cytometric immunophenotyping, a call will be made to the referring pathologist. The specimen can be snap-frozen for phenotyping by immunohistochemistry.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | |
External
link
PDF
document
Excel
document
Word
document