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Test ID: 5368    
Central Nervous System Consultation, Autopsy

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Specimen Type Describes the specimen type needed for testing

Tissue

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

It is imperative that detailed clinical information and, when possible, operative notes and radiographs accompany the specimen. Hematoxylin-and-eosin stained sections are also acceptable but must be accompanied by paraffin blocks and/or remaining wet tissue.

 

Forms:

1. If not ordering electronically, submit a Pathology/Cytology Request Form (Supply T246) with the specimen.

2. Submitting Brain/Spinal Cord for Examination in Suspected Neurodegenerative Disorders or Submitting Brain for Neuropathologic Examination-Cases Suspected of Creutzfeldt-Jakob Disease (CJD) in Special Instructions.

 

Upon completion of consultation, the brain tissue will be stored indefinitely in the Mayo Clinic Tissue Registry. The Mayo Clinic policy precludes our evaluation of cases under litigation that involve non-Mayo Clinic patients.

 

Preferred:                                                         

Specimen Type: Complete brain (and spinal cord when indicated)

Container/Tube: Container with 10% formalin and plastic bag

Specimen Volume: Entire brain

Collection Instructions:

1. Divide brain in half.

2. Place half the brain in 10% formalin if non-CJD, and 15% formalin for suspected cases of CJD, suspended by a thread under the basilar artery and fixed for 7 to 10 days.

3. Place other half of the brain into a plastic bag labeled with identifying information (ie, patient name, date of birth, autopsy number, and date of collection). Freeze and store in a -70 degrees C freezer.

4. Ship specimen using the Central Nervous System Consult Kit (Supply T633).

 

Acceptable:

Specimen Type: Tissue

Sources: Brain (and spinal cord when indicated)

Container/Tube: Plastic container

Specimen Volume: 1 cm(3) cube

Collection Instructions:

1. Take a small (1 cm[3]) cube of brain from 1 of the frontal lobes (typically, right inferior frontal).

2. Wrap in aluminum foil and place in plastic container.

3. Label container with identifying information (ie, patient name, date of birth, autopsy number, and date of collection).

4. Freeze and store in a -70 degrees C freezer.

5. Place the remaining brain in 10% formalin if non-CJD, and 15% formalin for suspected cases of CJD, suspended by a thread under the basilar artery and fixed for 7 to 10 days.

6. Ship specimen using the Central Nervous System Consult Kit (Supply T633).

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

NA

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Note: No specimen should be rejected. If specimen not received at appropriate temperature, include note to laboratory. If questions, contact laboratory.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
TissueAmbient (preferred)
 Frozen 
 Refrigerated