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| Web: | MayoMedicalLaboratories.com |
|---|---|
| Email: | mml@mayo.edu |
| Telephone: | 800.533.1710 |
| International: | 507.266.5700 |
| Values are valid only on day of printing. | |
Factors affecting specimen quality are as follows.
To optimize the study, we suggest special attention to the following:
1. Sampling: The specimen should be representative; thus, we
suggest reducing sampling error by obtaining tissue fragments
from several sites of the lesion.
2. Specimen size: Optimal preservation requires rapid fixation. In
view of the slow penetration of glutaraldehyde (1 mm/hour),
specimens should not exceed 1 mm in largest dimension.
3. Fixation: Glutaraldehyde or Trump's fixative (4% formaldehyde:
1% glutaraldehyde) is optimal. If no tissues fixed primarily in
glutaraldehyde are available, shavings can be taken from the
surface of tissues in formalin, where fixation was most rapidly
achieved. Paraffin-embedded tissues can be run-back to
aqueous-phase solution and processed for ultrastructural
study; however, in such preparations (paraffin-embedded and
formal fixed), fine structural detail invariably is poor. If the latter is
the only tissue available for study, we prefer that the paraffin
block be submitted.
All specimens should be accompanied by: a representative
hematoxylin-and-eosin-stained section and several unstained slides.
Such material permits additional studies which often serve to guide
the ultrastructural examination.
Note: Please complete a "Pathology/Dermatopathology
Request Form" or a "MayoConnect Additional Test
Information Form" (Supply T357) and forward it with the
specimen. These forms are available upon request from
Mayo Medical Laboratories. Requisitions for the procedure
cannot be processed unless the information requested is
supplied.
Ambient\Refrig NO\Frozen NO
Specimens Other Than:
Anticoagulants Other Than: NA
Hemolysis: NA
Thawing: N/A
Lipemia: NA
Monday through Thursday; Varies/am