Strict Criteria Sperm Morphology for Infertility Diagnosis and Treatment, Semen
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen Volume: 2 slides-10 microL of liquefied semen on each slide
1. Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
2. If sperm concentration is <10 million/mL, centrifuge the specimen at 300 x G for 10 minutes before making slides.
3. Label 2 frosted slides in pencil with the patient's first and last name and the date of specimen collection.
4. Allow the semen to liquefy for 30 minutes.
5. Place 10 microL of liquefied semen on the label end of each slide, and evenly smear the specimen using a plain slide (this process is the same as making a blood smear).
6. Allow the smears to air dry for 15 minutes before placing both slides into one slide holder for shipment.
Additional Information: Sperm concentration/mL is required (million/mL).
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
A minimum count is needed; lab will determine
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.