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Test Catalog

Test ID: EPFRP    
Bacterial Enteric Pathogens, Molecular Detection, PCR, Feces

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Shiga Toxin DNA is unlikely.


Forms: If not ordering electronically, complete, print, and send a Microbiology Request Form (T244) (


Specimen source is required.


Submit only 1 of the following specimens:



Specimen Type: Preserved Stool

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of stool

Collection Instructions: Collect fresh stool and submit in container with transport medium.



Specimen Type: Unpreserved Stool

Container/Tube: Stool container (Supply T288)

Specimen Volume: Representative portion of stool

Collection Instructions: Collect fresh stool and submit in container.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Formed stool or stool in gel transport medium

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
FecalAmbient (preferred)7 days
 Frozen 7 days
 Refrigerated 7 days