Test ID: LCMS
Leukemia/Lymphoma Immunophenotyping by Flow Cytometry
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen must arrive within 48 hours of collection for spinal fluid, 96 hours for peripheral blood and bone marrow, or 72 hours for fluids.
This test is not appropriate for and cannot support diagnosis of sarcoidosis, hypersensitivity pneumonitis, interstitial lung diseases, or differentiating between pulmonary tuberculosis and sarcoidosis (requests for CD4/CD8 ratios). Specimens sent for these purposes will be rejected.
-Bronchoalveolar lavage specimens submitted for evaluation for leukemia or lymphoma are appropriate to send for this test.
The following information is required:
1. Pertinent clinical history including reason for referral or clinical indication
2. Clinical or morphologic suspicion
3. Specimen source
4. Date and time of collection
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: ACD (solution A), heparin, EDTA
Specimen Volume: 10 mL
Collection Instructions:
1. Do not transfer blood to other containers.
2. Include 5- to 10-unstained blood smears, if possible.
3. Label specimen as blood.
Specimen Stability Information: Ambient <96 hours/Refrigerated < or =96 hours
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: ACD (solution A), heparin, EDTA
Specimen Volume: 1-5 mL
Collection Instructions:
1. Submission of bilateral specimens is not required.
2. Include 5- to 10-unstained bone marrow aspirate smears, if possible.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient <96 hours/Refrigerated < or =96 hours
Additional Information: If cytogenetic tests are also desired when drawing LCMS/3287 Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, an additional specimen should be submitted. It is important that the specimen be obtained, processed, and transported according to instructions for the other required test.
Specimen Type: Fluid
Sources: Serous effusions
Container/Tube: Body fluid container
Specimen Volume: 20 mL
Collection Instructions:
1. If possible, the fluids other than spinal fluid should be anticoagulated with heparin (1 U/mL of fluid).
2. The volume of fluid necessary to phenotype the lymphocytes or blasts in serous effusions depends upon the cell count in the specimen. Usually 20 mL of pleural or peritoneal fluid is sufficient. Smaller volumes can be used if there is a high cell count.
3. Label specimen with fluid type.
Specimen Stability Information: Refrigerated <72 hours/Ambient < or =72 hours
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 1-1.5 mL
Collection Instructions:
1. An original cytospin preparation (preferably unstained) must be included with the spinal fluid specimen so correlative morphologic evaluation can occur.
2. The volume of fluid necessary to phenotype the lymphocytes or blasts in spinal fluid depends upon the cell count in the specimen. A cell count should be determined and submitted with the specimen. Usually 1 to 1.5 mL of spinal fluid is sufficient. Smaller volumes can be used if there is a high cell count. If cell count is <10 cells/mcL, a larger volume of spinal fluid may be required. When cell counts drop below 5 cells/mcL, the immunophenotypic analysis may not be successful.
3. Label specimen as spinal fluid.
Specimen Stability Information: Refrigerated <48 hours/Ambient < or =48 hours
Additional Information: Spinal fluid cell and differential counts are required.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
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