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Test ID: PPFWE    
Protoporphyrins, Fractionation, Washed Erythrocytes

Available on the App Store

Specimen Type Describes the specimen type needed for testing

Washed RBC

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

All porphyrin tests on erythrocytes can be performed on 1 draw tube.

 

Collection Container/Tube:

Preferred: Green top (sodium heparin)

Acceptable: Metal free, lavender top (EDTA) or green top (lithium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: Washed erythrocyte suspension

Collection Instructions:

1. Patient should abstain from alcohol for 24 hours.

2. Collect and process whole blood specimen as follows:

a. Immediately place specimen on wet ice.

b. Transfer entire specimen to a 12-mL graduated centrifuge tube.

c. Centrifuge specimen for 10 minutes at 2,000 rpm.

d. Record volume of packed cells and the total volume of the specimen.

e. Discard supernatant plasma.

f. Wash packed erythrocytes 2 times by resuspension of at least an equal amount of cold 0.9% saline, mix, and centrifuge for 5 minutes at 2,000 rpm, discarding supernatant after each washing.

g. Resuspend packed cells to the original total volume with 0.9% saline. Invert specimen gently to mix.

Additional Information:

1. Volume of packed cells and total volume of erythrocyte suspension (red cells + saline) are required and must be sent with specimen.

2. Include a list of medications the patient is currently taking.

Forms:

1.     1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions.

2.     2. If not ordering electronically, submit a Biochemical Genetics Request Form (Supply T439) with the specimen.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

1 mL of washed and resuspended erythrocytes

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Cell suspension not available

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Washed RBCFrozen14 days