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Test ID: NKCP    
Natural Killer (NK) Cytotoxicity Profile

Specimen Type Describes the specimen type needed for testing

Control
WB Sodium Heparin
Whole Blood EDTA

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimens must arrive within 24 hours of draw. Specimens must arrive in the testing laboratory weekdays by 2 p.m. Specimens arriving on the weekend may be canceled. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (Supply T668).

 

Date and time of draw and ordering physician's name and phone number are required.

 

The MML Expedite procedure is recommended to ensure specimens are processed as quickly as possible once received at Mayo Medical Laboratories. Contact 800-533-1710 to add this procedure to your order. A nominal fee will be charged.

 

Three separate specimens (a whole blood EDTA specimen and a whole blood sodium heparin specimen in addition to a control specimen) are required.

 

Patient

Specimen Type: Whole blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 10 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. Label specimen as blood for NKC / Natural Killer Cell Cytotoxicity.

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Send specimen in original tube. Do not aliquot.

2. Label specimen as blood for TBBS / T- and B-Cell Quantitation by Flow Cytometry.

 

Normal Control

Specimen Type: Whole blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 10 mL

Collection Instructions:

1. Draw a control blood from a normal (healthy), unrelated person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Send specimen in original tube. Do not aliquot.

4. Rubberband with patient specimen.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Patient: 1 x 10 mL Sodium Heparin and 1 x 3 mL EDTA; Shipping Control: 1 x 10 mL Sodium Heparin

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

ACD or lithium heparin, specimens received in aliquot tubes

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
ControlAmbient24 hours
WB Sodium HeparinAmbient24 hours
Whole Blood EDTAAmbient52 hours