Test ID: NKCP
Natural Killer (NK) Cytotoxicity Profile
Specimen Type 
Describes the specimen type needed for testing
WB Sodium Heparin
Whole Blood EDTA
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimens must arrive within 24 hours of draw. Specimens must arrive in the testing laboratory weekdays by 2 pm; there is no weekend processing. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (Supply T668).
Date and time of draw and ordering physician's name and phone number are required.
Note: The MML Expedite procedure is recommended to ensure specimens are processed as quickly as possible once received at Mayo Medical Laboratories. Contact 800-533-1710 to add this procedure to your order. A nominal fee will be charged.
Three separate specimens (a whole blood EDTA specimen and a whole blood sodium heparin specimen in addition to a control specimen) are required.
Patient
Specimen Type: Whole blood for
Container/Tube: Green top (sodium heparin)
Specimen Volume: 10 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood for NKC/81733 Natural Killer Cell Cytotoxicity.
Specimen Type: Whole blood for TBBS/9336 T- and B-Cell Quantitation by Flow Cytometry
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label specimen as blood for TBBS/9336 T- and B-Cell Quantitation by Flow Cytometry.
Normal Control
Specimen Type: Whole blood for NKC/81733 Natural Killer Cell Cytotoxicity
Container/Tube: Green top (sodium heparin)
Specimen Volume: 10 mL
Collection Instructions:
1. Draw a control blood from a normal (healthy), unrelated person at the same time as the patient.
2. Label clearly on outermost label normal control.
3. Send specimen in original tube. Do not aliquot.
4. Rubberband with patient specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icterus | NA |
| Other | ACD or lithium heparin, specimens received in aliquot tubes |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Control | Ambient | 24 hours |
| WB Sodium Heparin | Ambient | 24 hours |
| Whole Blood EDTA | Ambient | 48 hours |
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