Test ID: CDFAW    
Chlamydia trachomatis, Fluorescence Staining of MicroTrak Slides

In suspected cases of child abuse, culture is the only recommended method of diagnosis.

Specimen source is required.

Submit only 1 of the following specimens:

Swab specimen must be collected using Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Type: Urethra (Males Only)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to collection.

2. Insert a small Dacron swab 2 to 4 cm into urethra.

3. Rotate swab and withdraw.

Slide preparation:

1. Slides made from swab specimens should be prepared immediately after specimen collection.

2. Firmly roll 1 side of swab over top half of well, then roll other side over bottom half. Cover entire well and stay within well perimeter.

3. Check coverage.

4. Allow specimen to completely air dry.

5. Lay slide flat; flood with 0.5 mL methanol fixative and let entire quantity evaporate. To speed evaporation, tip slide after 5 minutes to drain excess fixative.

6. For best results, store/transport either at room temperature (20-30 degrees C) or refrigerated at 2 to 8 degrees C, and stain within 7 days of collection. If not stained within 7 days, fixed specimen should be stored at -20 degrees C.

Specimen Type: Cervical (cytobrush)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. Wipe exocervix with cotton or Dacron swab to remove excess mucus. Dispose of swab.

2. Gently insert cytobrush into endocervical canal past squamocolumnar junction.

3. Leave in place 2 to 3 seconds.

4. Rotate cytobrush 1 full turn (360 degrees).

5. Withdraw cytobrush without touching any vaginal surfaces.

Slide preparation:

1. Slides made from cytobrush specimens should be prepared immediately after specimen collection.

2. Place the portion of the cytobrush containing the specimen across the center of the well.

3. Rotate and twist the brush, moving the brush back and forth across the well. Liquid clinging to the brush will disperse cells across the well.

4. Check coverage.

5. Allow specimen to completely air dry.

6. Lay slide flat; flood with 0.5 mL methanol fixative and let entire quantity evaporate. To speed evaporation, tip slide after 5 minutes to drain excess fixative.

7. For best results, store/transport either at room temperature (20-30 degrees C) or refrigerated at 2 to 8 degrees C, and stain within 7 days of collection. If not stained within 7 days, fixed specimen should be stored at -20 degrees C.

Additional Information: Performance studies using the cytobrush on pregnant patients have not been conducted.

Specimen Type: Cervical (swab)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. A Dacron swab should be used to sample pregnant patients or patients with a small cervical os.

2. Wipe exocervix with cotton or Dacron swab to remove excess mucus. Dispose of swab.

3. Insert large or small Dacron swab into endocervical canal until most of Dacron tip is not visible.

4. Rotate swab for 5 to 10 seconds inside endocervical canal.

5. Withdraw swab without touching any vaginal surfaces.

Specimen Type: Rectal (swab)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. Specimen should be collected only from symptomatic patients.

2. Insert a large Dacron swab approximately 3 cm into anal canal.

3. Move swab from side to side to sample crypts.

4. Withdraw swab. If fecal contamination occurs, discard swab and obtain another specimen.

Specimen Type: Conjunctival (swab)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. Specimen should be collected only from symptomatic patients.

2. Apply a topical proparacaine-based anesthetic to the eye or eyes (optional).

3. Using the small swab, thoroughly swab the inner surface of the lower, then upper eyelid. If samples are taken from both eyes, use the swab on the less affected eye first to avoid further contamination of that eye.

Specimen Type: Nasopharyngeal (swab or aspirate)

Container/Tube: Trinity Biotech MicroTrak Chlamydia trachomatis Specimen Collection Kit (Supply T176)

Specimen Volume: Swab

Collection Instructions:

1. Specimen should be collected only from symptomatic patients.

2. Collect specimen from the posterior nasopharynx by nasal swab or nasal aspirate using a standard collection method.

Slide preparation (nasal aspirate):

1. Vortex specimen gently to break up mucus.

2. Place 1 drop of the vortexed specimen on well. Cover entire well and stay within well perimeter.

3. Check coverage.

4. Allow specimen to completely air dry.

5. Lay slide flat; flood with 0.5 mL methanol fixative and let entire quantity evaporate. To speed evaporation, tip slide after 5 minutes to drain excess fixative.

6. For best results, store/transport either at room temperature (20-30 degrees C) or refrigerated at 2 to 8 degrees C, and stain within 7 days of collection. If not stained within 7 days, fixed specimen should be stored at -20 degrees C