Unit Code 110302:
Neisseria gonorrhoeae by Nucleic Acid Amplification
Specimen Required
Swab specimens must be collected using an Aptima
Collection Unisex Swab. These swabs are contained in the Aptima
collection kit. Collect and transport swab specimens as follows:
Endocervix (Females Only)
Collect specimen using Aptima Swab Collection System (Supply T583)
1. Use cleaning swab (white shaft) to remove excess mucus
from endocervix and discard.
2. Insert second swab (blue shaft) 1 to 1.5 cm into
endocervical canal and rotate gently for 30 seconds.
Avoid touching vaginal wall when removing swab.
3. Place second swab into transport tube provided in
collection kit. Snap off swab at score line so swab fits into
closed tube.
4. Cap tube securely, and label tube with patient's entire name,
and date and time of collection.
5. Send specimen refrigerated.
Note: Specimen source is required on request form for
processing.
Vaginal (Females Only)
Collect specimen using Aptima Swab Collection System (Supply T584)
1. Insert swab (blue shaft) about 5 cm past introitus and
rotate gently for 30 seconds.
2. Place swab into transport tube provided in collection kit.
Snap off swab at score line so swab fits into closed tube.
3. Cap tube securely, and label tube with patient's entire name,
and date and time of collection.
4. Send specimen refrigerated.
Note: Specimen source is required on request form for
processing.
Urethra (Males Only)
Collect specimen using Aptima Swab Collection System (Supply T583)
1. Patient should be instructed not to urinate for 1 hour prior to
sampling.
2. With a rotating movement, insert swab (blue shaft) 2 to 4 cm into
urethra.
3. Once inserted, rotate swab gently at least 1 full rotation using
sufficient pressure to ensure swab comes into contact with all
urethral surfaces. Allow swab to remain inserted for 2 to 3
seconds.
4. Place swab in transport tube provided in collection kit. Snap
off swab at score line so swab fits into closed tube.
5. Cap tube securely, and label tube with patient's entire name,
and date and time of collection.
6. Send specimen refrigerated.
Note: Specimen source is required on request form for
processing.
Urine (Males and Females)
Use Aptima Urine Specimen Transport Tube (Supply T582)
1. Patient should not have urinated for at least 1 hour prior to
specimen collection.
2. Patient should collect first portion (approximately 15 to 20 mL)
of a voided urine (first part of stream--not midstream) into a
screw-capped, sterile, plastic, preservative-free specimen
collection container.
3. Transfer 2 mL of urine into the urine specimen transport tube
using the disposable pipette provided within 24 hours of
collection. The correct volume of urine has been added when
the fluid level is between the black fill lines on the urine transport
tube.
4. Send urine transport tube refrigerated.
Note: Specimen source is required on request form for
processing.
Transport Temperature
Refrig\Frozen OK\Ambient OK
Reject Due To
Specimens Other Than: Urine (in APTIMA tube), Endocervix,
Vaginal, Urethral
Anticoagulants Other Than: APTIMA media
Hemolysis: N/A
Thawing: No
Lipemia: N/A
Day(s) and Time(s) Test Performed
Monday through Friday; 1st shift


