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Test ID: LEPTO    
Leptospira Antibody, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The assay is an indirect hemagglutination procedure. This indirect hemagglutination test uses human type "O" erythrocytes that have been coated (sensitized) with genus-specific leptospiral antigens derived from Leptospira biflexa strain Patoc 1. When a positive patient serum is incubated with these coated cells, the antigen-antibody reaction causes agglutination of the cells. The antibody titer is measured by testing serial dilutions of positive sera and recording the highest serum dilution that produces the agglutination reaction. Control cells (unsensitized erythrocytes) are also tested to screen for nonspecific reactivity. Both IgG and IgM class antibodies are detected in the assay, but are not distinguished. (Sulzer CR, Glosser JW, Rogers F, et al: Evaluation of an indirect hemagglutination test for the diagnosis of human leptospirosis. J Clin Microbiol 1975;2:218-221)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Wednesday, Friday; 9 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day if negative 2 days if positive

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

5 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester