The assay is an indirect hemagglutination procedure. This indirect hemagglutination test uses human type "O" erythrocytes that have been coated (sensitized) with genus-specific leptospiral antigens derived from Leptospira biflexa strain Patoc 1. When a positive patient serum is incubated with these coated cells, the antigen-antibody reaction causes agglutination of the cells. The antibody titer is measured by testing serial dilutions of positive sera and recording the highest serum dilution that produces the agglutination reaction. Control cells (unsensitized erythrocytes) are also tested to screen for nonspecific reactivity. Both IgG and IgM class antibodies are detected in the assay, but are not distinguished.(Sulzer CR, Glosser JW, Rogers F, et al: Evaluation of an indirect hemagglutination test for the diagnosis of human leptospirosis. J Clin Microbiol 1975;2:218-221)
Monday, Wednesday, Friday; 9 a.m.
1 day if negative 2 days if positive