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Unit Code 9405:
T3 (Triiodothyronine), Reverse, Serum

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Method Description

The rT3 measurement utilizes a standard radioimmunoassay procedure.

rT3 in the patient specimen, standard, or control competes with radio-

labeled rT3 for a limited number of binding sites on a specific antibody.

When the reaction period is complete, the antibody bound rT3 is

separated from the reaction mixture with a double antibody/

polyethylene glycol(PEG) precipitation.  After counting the bound counts

in a gamma-counter, a response curve is constructed in which the amount

of (125)I-rT3 bound is inversely proportional to the endogenous rT3 in the

specimen. (Package insert:  BioChem Immuno Systems, Bologna, Italy)

Performing Laboratory Location

Rochester

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