Unit Code 9405:
T3 (Triiodothyronine), Reverse, Serum
Method Description
The rT3 measurement utilizes a standard radioimmunoassay procedure.
rT3 in the patient specimen, standard, or control competes with radio-
labeled rT3 for a limited number of binding sites on a specific antibody.
When the reaction period is complete, the antibody bound rT3 is
separated from the reaction mixture with a double antibody/
polyethylene glycol(PEG) precipitation. After counting the bound counts
in a gamma-counter, a response curve is constructed in which the amount
of (125)I-rT3 bound is inversely proportional to the endogenous rT3 in the
specimen. (Package insert: BioChem Immuno Systems, Bologna, Italy)
Performing Laboratory Location
Rochester


