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Unit Code 9404:
T3 (Triiodothyronine), Free, Serum

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Method Description

The instrument used is a Beckman Coulter DxI 800. The Access Free

T3 assay is a competitive-binding immunoenzymatic assay. A sample

is added to a reaction vessel with an anti-T3 monoclonal antibody

conjugated to alkaline phosphatase. During the incubation, free T3 in

the sample reacts with the anti-T3 antibody. Particles coated with

streptavidin and biotinylated T3 analog are then added to the mixture.

Unoccupied binding sites on the anti-T3 antibody are bridged to the

particle through the T3 analog. After incubation in a reaction vessel,

materials bound to the solid phase are held in a magnetic field, while

unbound materials are washed away. Chemiluminescent substrate

Lumi-Phos 530 is added to the vessel and the light generated by the

reaction is measured with a luminometer. The light production is

inversely proportional to the concentration of free T3 in the sample.

The amount of analyte in the sample is determined from a stored,

multipoint calibration curve. (Beckman Coulter Assay Manual 2007)

Performing Laboratory Location

Rochester

Key