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Unit Code 9336:
T- and B-Cell Quantitation by Flow Cytometry

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Method Description

The T- and B-cell surface marker assay uses monoclonal antibodies

to identify the various membrane antigens, and flow cytometry to

enumerate the number of cells expressing these differentiation

antigens. The results are reported as the percent of lymphocytes

that are T-helper (CD3 , CD4 ), T-suppressor (CD3 , CD8 ), natural

killer (CD16 56 , CD3-), and B-lymphocytes (CD19 ), and the absolute

number of each cell type per mL of blood. The assay is a 4-color

no-wash procedure and the absolute counts are calculated from

internal bead standards. In addition, the total lymphocyte count and

the helper-suppressor ratio (T[H]/T[S]) is reported. (Hoffman RA,

Kung PC, Hansen WP, Goedstien G: Simple and rapid measurement

of human T lymphocytes and their subclasses in peripheral blood.

Proc Natl Acad Sci USA, 1980:77:4914-4917; U.S. Department of

Health and Human Services:  Guidelines for performance of

CD4 T-cell determinations in persons with human immunodeficiency

virus infection.  MMWR Morb Mortal Wkly Rep 1997;46 no. RR-2:pp 1-29)                            

Performing Laboratory Location

Rochester

Key