Intrinsic Factor Blocking Antibody, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The instrument used is a Beckman Coulter Unicel DXI 800. The Access Intrinsic Factor Antibody assay is a competitive binding immunoenzymatic assay. A sample is added to a reaction vessel along with intrinsic factor alkaline phosphatase conjugate and a protein blocking solution. Intrinsic factor antibody in the sample binds to the intrinsic factor conjugate. After incubation in a reaction vessel, paramagnetic particles coated with a mouse monoclonal, specific for the vitamin B12 binding site on intrinsic factor, is added to the reaction. Intrinsic factor conjugate that has not been blocked by sample anti-intrinsic factor binds to the monoclonal antibody on the solid phase. After an additional incubation in the reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Chemiluminescent substrate is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is inversely proportional to the concentration of intrinsic factor antibody in the sample expressed in AU/mL (Antibody Units/mL). The amount of analyte in the sample is determined from a stored calibration. (Beckman Coulter Assay Manual 2009, Beckman Coulter Inc., Fullerton, CA)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-12 p.m., Saturday; 6 a.m.-6 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test