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Test ID: CA25    
Cancer Antigen 125 (CA 125), Serum

Method Description Describes how the test is performed and provides a method-specific reference

The instrument used is a Beckman Coulter DXI 800. The Access OV Monitor assay is a two-site immunoenzymatic "sandwich" assay. A sample is added to a reaction vessel along with mouse monoclonal anti-CA 125 antigen alkaline phosphatase conjugate and paramagnetic particles coated with a second mouse monoclonal anti-CA 125 antigen antibody. The CA 125 antigen in the sample binds to the immobilized monoclonal anti-CA 125 antigen on the solid phase, while the conjugate antibody reacts with a different antigenic site on the CA 125 antigen molecule. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of CA 125 antigen in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve. (Package insert: OV Monitor Reagent Pack, Beckman Coulter Inc., Brea, CA 2010)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 5 a.m.-12 a.m.

Saturday; 6 a.m.-6 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 months

Performing Laboratory Location The location of the laboratory that performs the test

Rochester