Sex Hormone-Binding Globulin (SHBG), Serum
Method Description Describes how the test is performed and provides a method-specific reference
IMMULITE 2000 sex hormone-binding globulin (SHBG) is a solid-phase, 2-site chemiluminescent immunoassay. A polystyrene bead is coated with a monoclonal antibody specific for sex hormone-binding globulin (SHBG). The patient sample, diluted 1 in 20, and alkaline phosphatase-conjugated anti-SHBG (polyclonal) are added to the bead and incubated. SHBG in the sample is bound to form an antibody sandwich complex. Unbound conjugate is then removed by washing, after which substrate is added. The chemiluminescent substrate, a phosphate ester of adamantly dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of this substance results in the sustained emission of light. The photon output is proportional to the concentration of SHBG in the sample.(Package insert: IMMULITE SHBG 2000, July 2008)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test