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Test ID: HIAA
5-Hydroxyindoleacetic Acid (5-HIAA), Urine

Method Description Describes how the test is performed and provides a method-specific reference

5-Hydroxyindoleacetic acid (5-HIAA) is measured by C18 solid phase extraction (SPE) of an aliquot from a 24-hour urine collection and liquid chromatography/tandem mass spectrometry analysis using an amide-C16 high performance liquid chromatography column at a flow rate of 1.0 mL/min. 5-HIAA elutes apart from the bulk of the specimen matrix at a retention time of approximately 1.5 minutes. 5-HIAA is quantitated using a custom synthesized stable isotope labeled internal standard (d6-5-HIAA) from calibration over a concentration range 0.5 to 150 mg/L. (Kroll CA, Magera MJ, Helgeson JK, et al: A liquid chromatography-tandem mass spectrometry method for the determination of 5-hydroxyindole-3-acetic acid in urine. Clin Chem 2002;48:2049-2051)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 11 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days (not reported on Saturday or Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester