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Unstained whole blood is first examined microscopically in search of motile microfilariae. Portions of the specimen are concentrated by centrifugation after adding 2% formalin. The sediment is examined as a wet preparation, and, if positive, the slide is air dried, fixed in methyl alcohol, and stained with Giemsa to aid in species identification.(Garcia L, Bruckner D: Diagnostic Medical Parasitology. 3rd edition. Washington, DC, American Society for Microbiology, 1997)
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