Organism Referred for Identification, Aerobic Bacteria
Method Description Describes how the test is performed and provides a method-specific reference
Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram-stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, routine media utilized includes chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35 degrees C in 5% CO2, the organism is identified using 1 or a combination of the following techniques: commercial identification strips or panels, MALDI-TOF mass spectrometry, conventional biochemical tests, carbon source utilization, and sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.(Manual of Clinical Microbiology. Seventh edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al. Washington, DC, ASM Press, 1999; RS Weyant, CW Moss, RE Weaver, et al: Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria. CDC, Atlanta, GA, 1996; Bergey's Manual of Systematic Bacteriology, Vol 1. Edited by NR Krieg. Baltimore, MD, Williams and Wilkins, 1984; Kolbert CP, Persing DH: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 1999;2:299-305)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
2 days/1 month if on slant
Performing Laboratory Location The location of the laboratory that performs the test