Test ID: SEMA
Semen Analysis with WHO Morphology
Method Description
Describes how the test is performed and provides a method-specific reference
Semen Analysis:
Semen is incubated for up to 1 hour at body temperature (37 degrees C) to allow the sample to liquefy. The sample is then measured for volume and analyzed microscopically to determine the number of sperm present, the number of moving or motile sperm, and the properties of the sperm motility.(The World Health Organization [WHO] Laboratory Manual for the examination of human semen and sperm-cervical mucus interaction. Fifth edition. Cambridge University Press, 2010)
WHO Morphology:
In addition, a drop of semen is spread on a microscope slide. The slide is then processed with stains that give different colors to different parts of the sperm. Using high-power microscopy, the shape and size of at least 100 sperm are compared to a "normal" reference sperm. The semen parameters measured in the WHO morphology include normal oval, head abnormalities, and the presence of other cellular elements.(The World Health Organization [WHO] Laboratory Manual for the examination of human semen and sperm-cervical mucus interaction. Third edition. Cambridge University Press, 1992)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 3 p.m.
Send specimen Monday through Thursday only and not the day before a holiday.


